Preliminary observations on whole-ovary xenotransplantation as an experimental model for fertility preservation.

Autor: Nichols-Burns SM; Department of Obstetrics and Gynecology, Erlangen University Hospital, University of Erlangen-Nuremberg, Universitätsstrasse 21-23, Erlangen 91054, Germany., Lotz L; Department of Obstetrics and Gynecology, Erlangen University Hospital, University of Erlangen-Nuremberg, Universitätsstrasse 21-23, Erlangen 91054, Germany., Schneider H; Department of Obstetrics and Gynecology, Erlangen University Hospital, University of Erlangen-Nuremberg, Universitätsstrasse 21-23, Erlangen 91054, Germany., Adamek E; Department of Heart Surgery, Erlangen University Hospital, University of Erlangen-Nuremberg, Universitätsstrasse 21-23, Erlangen 91054, Germany., Daniel C; Department of Nephropathology, Erlangen University Hospital, University of Erlangen-Nuremberg, Universitätsstrasse 21-23, Erlangen 91054, Germany., Stief A; Department of Nephropathology, Erlangen University Hospital, University of Erlangen-Nuremberg, Universitätsstrasse 21-23, Erlangen 91054, Germany., Grigo C; Department of Nephropathology, Erlangen University Hospital, University of Erlangen-Nuremberg, Universitätsstrasse 21-23, Erlangen 91054, Germany., Klump D; Department of Plastics and Hand Surgery, Erlangen University Hospital, University of Erlangen-Nuremberg, Universitätsstrasse 21-23, Erlangen 91054, Germany., Hoffmann I; Department of Obstetrics and Gynecology, Erlangen University Hospital, University of Erlangen-Nuremberg, Universitätsstrasse 21-23, Erlangen 91054, Germany., Beckmann MW; Department of Obstetrics and Gynecology, Erlangen University Hospital, University of Erlangen-Nuremberg, Universitätsstrasse 21-23, Erlangen 91054, Germany., Dittrich R; Department of Obstetrics and Gynecology, Erlangen University Hospital, University of Erlangen-Nuremberg, Universitätsstrasse 21-23, Erlangen 91054, Germany. Electronic address: ralf.dittrich@uk-erlangen.de.
Jazyk: angličtina
Zdroj: Reproductive biomedicine online [Reprod Biomed Online] 2014 Nov; Vol. 29 (5), pp. 621-6. Date of Electronic Publication: 2014 Aug 12.
DOI: 10.1016/j.rbmo.2014.07.017
Abstrakt: Ovarian tissue preservation and retransplantation is a promising strategy to restore fertility in cancer survivors. Ischaemia accompanying ovarian tissue grafting, however, can lead to significant follicle loss. Transplantation of the whole ovary by vascular anastomosis has been considered as an alternative to prevent widespread ischaemic damage. In this study, the feasibility and function of transplanting whole ovary with intact vasculature were evaluated, with the goal of developing a xenograft model for studies using donated human ovaries. Whole-swine ovaries with vascular pedicles were perfused and transplanted as intact ovaries by anastomosis into irradiated ovariectomized nude rats (n = 10). The observation period was between 1 and 4 weeks. Fresh swine ovaries served as controls (n = 10). Ovarian stroma and follicle populations were assessed through histological examination in both transplanted and control ovaries. Most of the transplanted whole ovaries (n = 6) maintained stromal quality and all preantral follicle classes were represented, although follicle numbers decreased compared with fresh control. Four transplanted ovaries were fibrotic after 1-4 weeks within the nude rat. Our results demonstrate transplantation of whole-pig ovary into nude rats is possible and support development of this xenograft model system for human studies.
(Copyright © 2014 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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