Bacterial noncoding Y RNAs are widespread and mimic tRNAs.

Autor:	Chen X; Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06510, USA., Sim S; Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06510, USA., Wurtmann EJ; Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06510, USA., Feke A; Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06510, USA., Wolin SL; Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06510, USA Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06510, USA sandra.wolin@yale.edu.
Jazyk:	angličtina
Zdroj:	RNA (New York, N.Y.) [RNA] 2014 Nov; Vol. 20 (11), pp. 1715-24. Date of Electronic Publication: 2014 Sep 17.
DOI:	10.1261/rna.047241.114
Abstrakt:	Many bacteria encode an ortholog of the Ro60 autoantigen, a ring-shaped protein that is bound in animal cells to noncoding RNAs (ncRNAs) called Y RNAs. Studies in Deinococcus radiodurans revealed that Y RNA tethers Ro60 to polynucleotide phosphorylase, specializing this exoribonuclease for structured RNA degradation. Although Ro60 orthologs are present in a wide range of bacteria, Y RNAs have been detected in only two species, making it unclear whether these ncRNAs are common Ro60 partners in bacteria. In this study, we report that likely Y RNAs are encoded near Ro60 in >250 bacterial and phage species. By comparing conserved features, we discovered that at least one Y RNA in each species contains a domain resembling tRNA. We show that these RNAs contain nucleotide modifications characteristic of tRNA and are substrates for several enzymes that recognize tRNAs. Our studies confirm the importance of Y RNAs in bacterial physiology and identify a new class of ncRNAs that mimic tRNA. (© 2014 Chen et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.)
Databáze:	MEDLINE
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