| Autor: |
Hung AH; Department of Chemistry, Molecular Biosciences, Neurobiology, Biomedical Engineering, and Radiology, Northwestern University , 2145 Sheridan Road, Evanston, Illinois 60208-3113, United States., Holbrook RJ, Rotz MW, Glasscock CJ, Mansukhani ND, MacRenaris KW, Manus LM, Duch MC, Dam KT, Hersam MC, Meade TJ |
| Jazyk: |
angličtina |
| Zdroj: |
ACS nano [ACS Nano] 2014 Oct 28; Vol. 8 (10), pp. 10168-77. Date of Electronic Publication: 2014 Sep 19. |
| DOI: |
10.1021/nn502986e |
| Abstrakt: |
The delivery of bioactive molecules into cells has broad applications in biology and medicine. Polymer-modified graphene oxide (GO) has recently emerged as a de facto noncovalent vehicle for hydrophobic drugs. Here, we investigate a different approach using native GO to deliver hydrophilic molecules by co-incubation in culture. GO adsorption and delivery were systematically studied with a library of 15 molecules synthesized with Gd(III) labels to enable quantitation. Amines were revealed to be a key chemical group for adsorption, while delivery was shown to be quantitatively predictable by molecular adsorption, GO sedimentation, and GO size. GO co-incubation was shown to enhance delivery by up to 13-fold and allowed for a 100-fold increase in molecular incubation concentration compared to the alternative of nanoconjugation. When tested in the application of Gd(III) cellular MRI, these advantages led to a nearly 10-fold improvement in sensitivity over the state-of-the-art. GO co-incubation is an effective method of cellular delivery that is easily adoptable by researchers across all fields. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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