| Autor: |
Schroer TA; Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110., Steuer ER, Sheetz MP |
| Jazyk: |
angličtina |
| Zdroj: |
Cell [Cell] 1989 Mar 24; Vol. 56 (6), pp. 937-46. |
| DOI: |
10.1016/0092-8674(89)90627-2 |
| Abstrakt: |
The role of cytoplasmic dynein in microtubule-based organelle transport was examined using a reconstituted assay developed from chick embryo fibroblasts. Factors present in a high-speed cytosol caused the movement of purified organelles on microtubules predominantly in the minus end direction. Inactivation of cytoplasmic dynein in the high-speed cytosol by vanadate-mediated UV photocleavage inhibited minus end-directed organelle motility by over 90%. Addition of purified cytoplasmic dynein to the inactive cytosol restored minus end-directed organelle motility, although purified cytoplasmic dynein by itself did not support organelle movement. We propose that cytoplasmic dynein is the motor for minus end-directed organelle movement, but that additional cytosolic factors are also required to produce organelle motility. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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