The Low Prevalence of Y Chromosomal Microdeletions is Observed in the Oligozoospermic Men in the Area of Mato Grosso State and Amazonian Region of Brazilian Patients.

Autor:	Dos Santos Godoy GC; Department of Basic Sciences, Medical School, UFMT, Cuiabá, MT, Brazil., Galera BB; Department of Basic Sciences, Medical School, UFMT, Cuiabá, MT, Brazil., Araujo C; Faculty of Biology, Federal University of Mato Grosso, UFMT, Cuiabá, MT, Brazil., Barbosa JS; Tropical Institute of Reproductive Medicine and Menopause, Cuiabá, MT, Brazil., de Pinho MF; Faculty of Biology, Federal University of Mato Grosso, UFMT, Cuiabá, MT, Brazil., Galera MF; Department of Basic Sciences, Medical School, UFMT, Cuiabá, MT, Brazil., de Medeiros SF; Department of Gynecology and Obstetrics, Faculty of Medicine, UFMT, Cuiabá, MT, Brazil. ; Tropical Institute of Reproductive Medicine and Menopause, Cuiabá, MT, Brazil.
Jazyk:	angličtina
Zdroj:	Clinical medicine insights. Reproductive health [Clin Med Insights Reprod Health] 2014 Aug 11; Vol. 8, pp. 51-7. Date of Electronic Publication: 2014 Aug 11 (Print Publication: 2014).
DOI:	10.4137/CMRH.S15475
Abstrakt:	Objective: To determine the prevalence of chromosomal abnormalities and microdeletions on Y chromosome in infertile patients with oligozoospermia or azoospermia in Mato Grosso state, Brazil. Methods: This cross-sectional study enrolled 94 men from infertile couples. Karyotype analysis was performed by lymphocyte culture technique. DNA from each sample was extracted using non-enzymatic method. Microdeletions were investigated by polymerase chain reaction (PCR). Results: With the use of cytogenetic analysis, five patients (5.3%) had abnormal karyotype, one azoospermic patient (1.1%) had karyotype 46,XY,t(7;1) (qter-p35), one (1.1%) with mild oligozoospermia had karyotype 46,XY,delY(q), and two other azoospermic patients had karyotype 47,XXY, consistent with Klinefelter syndrome (KS). One of them (1.1%) with severe oligozoospermia had karyotype 46,XY,8p+. Microdeletion on Y chromosome was found in the azoospermia factor c (AZFc) region in only one azoospermic patient (1.1%). Conclusions: The prevalence of genetic abnormalities in oligo/azoospermic Brazilian men from infertile couple was 5.3%, and microdeletion on Y chromosome was not a common finding in this population (1.1%).
Databáze:	MEDLINE
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