Increase in the expression of CD4 + CD25+ lymphocytic T cells in the indeterminate clinical form of human Chagas disease after stimulation with recombinant antigens of Trypanosoma cruzi.

Autor: de Moura Braz SC; Departamento de Imunologia, Programa Integrado de Doença de Chagas/Fiocruz, Rio de Janeiro, Brazil., de Melo AS, da Glória Aureliano de Melo Cavalcanti M, Martins SM, de Oliveira W Jr, da Silva ED, Ferreira AG, de Lorena VM, de Miranda Gomes Y
Jazyk: angličtina
Zdroj: Journal of clinical immunology [J Clin Immunol] 2014 Nov; Vol. 34 (8), pp. 991-8. Date of Electronic Publication: 2014 Sep 10.
DOI: 10.1007/s10875-014-0092-6
Abstrakt: Purpose: Regulatory T cells are involved in the clinical course of chronic Chagas disease, possibly because they exercise a control in the patient's inflammatory response to Trypanosoma cruzi. This study analyzed the levels of CD4 + CD25+ T cells in chronic Chagas disease patients after in vitro stimulation of the peripheral blood mononuclear cells with CRA (Cytoplasmic Repetitive Antigen) or FRA (Flagellar Repetitive Antigen) T. cruzi antigens.
Methods: Groups of patients with the cardiac form and indeterminate form; and non-infected individuals, were selected. The CD4 + CD25+ T lymphocyte population, as well as the FoxP3 expression and the IL10 production, were evaluated by flow cytometry after stimulation with CRA or FRA.
Result: The IND group presented higher levels of CD4 + CD25+ T cells than the CARD group. However, there was no evidence of a relationship between FoxP3 and IL10 with any of the chronic forms.
Conclusions: Our results suggest the possible involvement of CD4 + CD25+ T cells specific to CRA and FRA in controlling the progression of clinical outcomes. Though, further studies are needed to define which mechanisms activate regulatory T cells and lead to pathology control in chronic human Chagas disease.
Databáze: MEDLINE
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