Successful live birth from oocytes after more than 14 years of cryopreservation.

Autor: Urquiza MF; Halitus, Instituto Médico, Capital Federal, Buenos Aires, Argentina, fernanda.urquiza@halitus.com., Carretero I, Cano Carabajal PR, Pasqualini RA, Felici MM, Pasqualini RS, Quintans CJ
Jazyk: angličtina
Zdroj: Journal of assisted reproduction and genetics [J Assist Reprod Genet] 2014 Nov; Vol. 31 (11), pp. 1553-5. Date of Electronic Publication: 2014 Sep 10.
DOI: 10.1007/s10815-014-0318-9
Abstrakt: Objective: To report a birth of a healthy girl after long-term oocyte cryopreservation by slow cooling in sodium depleted medium.
Design: Clinical application.
Setting: University Affiliated, Private IVF center.
Patient: A 38-year-old woman received embryos from IVF by intracytoplasmic sperm injection (ICSI) with her own oocytes that were cryopreserved by slow freezing in a low-sodium medium 14 years and 6 months before, when she was 24 years old.
Main Outcome Measure(s): Survival, fertilization, cleavage, clinical pregnancy and delivery.
Result(s): From six metaphase-II oocytes thawed, two survived, one was fertilized after ICSI and a cleaving embryo was transferred on day 3. A single term pregnancy was achieved, ending with the delivery of a healthy girl.
Conclusion(s): Cryopreservation after slow freezing in a sodium depleted medium maintained the developmental competence of oocytes after long-term storage and resulted in a successful live birth. As far as is known, this case represents, up to date, the longest storage period of cryopreserved human oocytes resulting in a live birth.
Databáze: MEDLINE
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