Development of 16 microsatellite markers within the Camassia (Agavaceae) species complex and amplification in related taxa.

Autor: Culley TM; Department of Biological Sciences, University of Cincinnati, 614 Rieveschl Hall, Cincinnati, Ohio 45221-0006 USA., Leng JF; Department of Biology, Willamette University, 900 State Street, Salem, Oregon 97301 USA., Kephart SR; Department of Biology, Willamette University, 900 State Street, Salem, Oregon 97301 USA., Cartieri FJ; Department of Biological Sciences, University of Cincinnati, 614 Rieveschl Hall, Cincinnati, Ohio 45221-0006 USA., Theiss KE; Department of Biology, Willamette University, 900 State Street, Salem, Oregon 97301 USA.
Jazyk: angličtina
Zdroj: Applications in plant sciences [Appl Plant Sci] 2013 Jul 22; Vol. 1 (8). Date of Electronic Publication: 2013 Jul 22 (Print Publication: 2013).
DOI: 10.3732/apps.1300001
Abstrakt: Premise of the Study: The North American genus Camassia is an ecologically important group whose variability and evolution are little understood, being influenced by hybridization and geographic isolation. We developed microsatellite markers to investigate patterns of gene flow, population structure, and taxonomic relationships within this group. •
Methods and Results: Using a traditional approach with biotin-labeled probes, we developed 16 microsatellite primers in three species of Camassia: C. howellii, C. leichtlinii, and C. quamash. The number of alleles per locus averaged 3.94 per species, and levels of heterozygosity ranged from 0.000 to 1.00 and 0.033 to 0.917 for observed and expected heterozygosities, respectively. All primers amplified to varying extents in additional species (C. angusta, C. cusickii, C. scilloides) and in putative species in a related genus (Hastingsia alba, H. atropurpurea, H. bracteosa, H. serpentinicola). •
Conclusions: These microsatellite markers exhibit variation and are useful for ongoing studies of integrative taxonomy and population differentiation within this species complex.
Databáze: MEDLINE
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