Antifungal susceptibility and virulence attributes of animal-derived isolates of Candida parapsilosis complex.

Autor:	Brilhante RSN; Department of Pathology and Legal Medicine, College of Medicine, Postgraduate Program in Medical Microbiology, Specialized Medical Mycology Center, Federal University of Ceará, Fortaleza, Ceará, Brazil., Rodrigues TJS; Department of Pathology and Legal Medicine, College of Medicine, Postgraduate Program in Medical Microbiology, Specialized Medical Mycology Center, Federal University of Ceará, Fortaleza, Ceará, Brazil., Castelo-Branco DSCM; Department of Pathology and Legal Medicine, College of Medicine, Postgraduate Program in Medical Microbiology, Specialized Medical Mycology Center, Federal University of Ceará, Fortaleza, Ceará, Brazil., Teixeira CEC; Department of Pathology and Legal Medicine, College of Medicine, Postgraduate Program in Medical Microbiology, Specialized Medical Mycology Center, Federal University of Ceará, Fortaleza, Ceará, Brazil., Macedo RB; Department of Pathology and Legal Medicine, College of Medicine, Postgraduate Program in Medical Microbiology, Specialized Medical Mycology Center, Federal University of Ceará, Fortaleza, Ceará, Brazil., Bandeira SP; Department of Pathology and Legal Medicine, College of Medicine, Postgraduate Program in Medical Microbiology, Specialized Medical Mycology Center, Federal University of Ceará, Fortaleza, Ceará, Brazil., Pereira de Alencar L; College of Veterinary Medicine, Postgraduate Program in Veterinary Sciences, State University of Ceará, Fortaleza, Ceará, Brazil.; Department of Pathology and Legal Medicine, College of Medicine, Postgraduate Program in Medical Microbiology, Specialized Medical Mycology Center, Federal University of Ceará, Fortaleza, Ceará, Brazil., Monteiro AJ; Department of Statistics and Applied Mathematics, Federal University of Ceará, Fortaleza, Ceará, Brazil., Cordeiro RA; Department of Pathology and Legal Medicine, College of Medicine, Postgraduate Program in Medical Microbiology, Specialized Medical Mycology Center, Federal University of Ceará, Fortaleza, Ceará, Brazil., Bandeira TJPG; School of Medicine, Christus College (Unichristus), Fortaleza, Ceará, Brazil.; Department of Pathology and Legal Medicine, College of Medicine, Postgraduate Program in Medical Microbiology, Specialized Medical Mycology Center, Federal University of Ceará, Fortaleza, Ceará, Brazil., Moreira JLB; Department of Pathology and Legal Medicine, College of Medicine, Postgraduate Program in Medical Microbiology, Specialized Medical Mycology Center, Federal University of Ceará, Fortaleza, Ceará, Brazil., Sidrim JJC; Department of Pathology and Legal Medicine, College of Medicine, Postgraduate Program in Medical Microbiology, Specialized Medical Mycology Center, Federal University of Ceará, Fortaleza, Ceará, Brazil., Rocha MFG; College of Veterinary Medicine, Postgraduate Program in Veterinary Sciences, State University of Ceará, Fortaleza, Ceará, Brazil.; Department of Pathology and Legal Medicine, College of Medicine, Postgraduate Program in Medical Microbiology, Specialized Medical Mycology Center, Federal University of Ceará, Fortaleza, Ceará, Brazil.
Jazyk:	angličtina
Zdroj:	Journal of medical microbiology [J Med Microbiol] 2014 Nov; Vol. 63 (Pt 11), pp. 1568-1572. Date of Electronic Publication: 2014 Sep 04.
DOI:	10.1099/jmm.0.076216-0
Abstrakt:	This study aimed to identify strains of the Candida parapsilosis complex isolated from animals, as well as to assess their in vitro antifungal susceptibility profile and in vitro production of virulence attributes. We used 28 isolates of C. parapsilosis sensu lato recovered from clinically healthy animals. The strains were characterized phenotypically, followed by molecular identification of the species through PCR-restriction enzyme analysis. The susceptibility of the strains to amphotericin B, itraconazole, voriconazole, fluconazole and caspofungin was assessed through broth microdilution. Additionally, the ability of the strains to produce biofilm, phospholipases and proteases was analysed. Molecular analysis showed 13 C. parapsilosis sensu stricto, 10 Candida orthopsilosis and five Candida metapsilosis strains. In vitro resistance to fluconazole was observed in three strains of C. parapsilosis sensu stricto and two C. metapsilosis. All tested strains were able to form biofilms and 23/28 isolates presented protease production, whilst none was able to produce phospholipases. Our study showed that C. parapsilosis sensu stricto and C. orthopsilosis are the most common species of the C. parapsilosis species complex and that these cryptic species present no significant phenotypical differences. (© 2014 The Authors.)
Databáze:	MEDLINE
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