Antisickling activity of butyl stearate isolated from Ocimum basilicum (Lamiaceae).

Autor:	Tshilanda DD; Faculté des Sciences B.P. 190, Université de Kinshasa, Kinshasa XI, R.D. Congo., Mpiana PT; Faculté des Sciences B.P. 190, Université de Kinshasa, Kinshasa XI, R.D. Congo., Onyamboko DN; Faculté des Sciences B.P. 190, Université de Kinshasa, Kinshasa XI, R.D. Congo., Mbala BM; Faculté des Sciences B.P. 190, Université de Kinshasa, Kinshasa XI, R.D. Congo., Ngbolua KT; Faculté des Sciences B.P. 190, Université de Kinshasa, Kinshasa XI, R.D. Congo., Tshibangu DS; Faculté des Sciences B.P. 190, Université de Kinshasa, Kinshasa XI, R.D. Congo., Bokolo MK; Faculté des Sciences B.P. 190, Université de Kinshasa, Kinshasa XI, R.D. Congo., Taba KM; Faculté des Sciences B.P. 190, Université de Kinshasa, Kinshasa XI, R.D. Congo., Kasonga TK; Institut Supérieur Pédagogique de la Gombe (ISP-Gombe), Kinshasa, R.D. Congo.
Jazyk:	angličtina
Zdroj:	Asian Pacific journal of tropical biomedicine [Asian Pac J Trop Biomed] 2014 May; Vol. 4 (5), pp. 393-8.
DOI:	10.12980/APJTB.4.2014C1329
Abstrakt:	Objective: To perform phytochemical analyses on the leaves of Ocimum basilicum L. (O. basilicum), to elucidate the structure of isolate and then perform the antisickling activity on the crude extract and on the isolate. Methods: The Emmel test performed on the acidified methanolic extract of this plant was used to evaluate the antisickling activity. The structure characterization of the active compound was performed using chromatographic techniques for the separation and the spectroscopic ones for structure elucidation (1H-NMR, 13C-NMR, COSY, HMBC). Results: The chemical screening on the crude extract revealed the presence of polyphenols (flavonoids, anthocyanins, leucoanthocyanins, tannins, quinones) alkaloids, saponins, triterpenoids and steroids. The obtained extract after evaporation yielded 34.50 g (11.5%) out of 300 g of powdered leaves of O. basilicum. The acidified methanolic extract and butyl stearate showed an interesting antisickling activity. Conclusions: The acidified methanolic extract and butyl stearate from O. basilicum displayed a good antisickling activity. To the best of our knowledge, this is the first time to report the antisickling activity of this compound in this plant. The synthesized compound presented the same spectroscopic characteristics than the natural one and the antisickling activities of its derivatives are understudying.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu