Bicaudal d family adaptor proteins control the velocity of Dynein-based movements.

Autor:	Schlager MA; Department of Neuroscience, Erasmus Medical Center, Rotterdam, the Netherlands., Serra-Marques A; Cell Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands., Grigoriev I; Cell Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands., Gumy LF; Cell Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands., Esteves da Silva M; Cell Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands., Wulf PS; Cell Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands., Akhmanova A; Cell Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands. Electronic address: a.akhmanova@uu.nl., Hoogenraad CC; Department of Neuroscience, Erasmus Medical Center, Rotterdam, the Netherlands; Cell Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands. Electronic address: c.hoogenraad@uu.nl.
Jazyk:	angličtina
Zdroj:	Cell reports [Cell Rep] 2014 Sep 11; Vol. 8 (5), pp. 1248-56. Date of Electronic Publication: 2014 Aug 28.
DOI:	10.1016/j.celrep.2014.07.052
Abstrakt:	Cargo transport along microtubules is driven by the collective function of microtubule plus- and minus-end-directed motors (kinesins and dyneins). How the velocity of cargo transport is driven by opposing teams of motors is still poorly understood. Here, we combined inducible recruitment of motors and adaptors to Rab6 secretory vesicles with detailed tracking of vesicle movements to investigate how changes in the transport machinery affect vesicle motility. We find that the velocities of kinesin-based vesicle movements are slower and more homogeneous than those of dynein-based movements. We also find that Bicaudal D (BICD) adaptor proteins can regulate dynein-based vesicle motility. BICD-related protein 1 (BICDR-1) accelerates minus-end-directed vesicle movements and affects Rab6 vesicle distribution. These changes are accompanied by reduced axonal outgrowth in neurons, supporting their physiological importance. Our study suggests that adaptor proteins can modulate the velocity of dynein-based motility and thereby control the distribution of transport carriers. (Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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