A fluorescent aptasensor for potassium ion detection-based triple-helix molecular switch.

Autor: Verdian-Doghaei A; Biophysical Chemistry Laboratory, Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran. Electronic address: asma_verdian@yahoo.com., Housaindokht MR; Biophysical Chemistry Laboratory, Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran., Abnous Kh; Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Jazyk: angličtina
Zdroj: Analytical biochemistry [Anal Biochem] 2014 Dec 01; Vol. 466, pp. 72-5. Date of Electronic Publication: 2014 Aug 28.
DOI: 10.1016/j.ab.2014.08.014
Abstrakt: Here, a biosensor based on a quadruplex-forming aptamer for the determination of potassium ion (K(+)) is presented. The aptamer was used as a molecular recognition element; it was adjacent to two arm fragments and a dual-labeled oligonucleotide serving as a signal transduction probe (STP) that is complementary of the arm fragment sequence. In the presence of K(+), the aptamer was displaced from the STP, which was accompanied by decreased signal. The quenching percentage of fluorescence intensity was proportional to the concentration of K(+) in the range of 0.05 to 1.4mM. A detection limit of 0.014 mM was achieved. Furthermore, other metal ions, such as Na(+), Li(+), NH4(+), Mg(2+), and Ca(2+), caused no notable interference on the detection of K(+).
(Copyright © 2014 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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