Cyclic peptide-capped gold nanoparticles for enhanced siRNA delivery.
| Autor: | Shirazi AN; School of Pharmacy, Chapman University, Irvine, CA 92618, USA., Paquin KL; Department of Cell and Molecular Biology, University of Rhode Island, Kingston, RI 02881, USA., Howlett NG; Department of Cell and Molecular Biology, University of Rhode Island, Kingston, RI 02881, USA., Mandal D; School of Pharmacy, Chapman University, Irvine, CA 92618, USA., Parang K; School of Pharmacy, Chapman University, Irvine, CA 92618, USA. parang@chapman.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Molecules (Basel, Switzerland) [Molecules] 2014 Aug 28; Vol. 19 (9), pp. 13319-31. Date of Electronic Publication: 2014 Aug 28. |
| DOI: | 10.3390/molecules190913319 |
| Abstrakt: | Previously, we have reported the synthesis of a homochiral l-cyclic peptide [WR]5 and its use for delivery of anti-HIV drugs and biomolecules. A physical mixture of HAuCl4 and the peptide generated peptide-capped gold nanoparticles. Here, [WR]5 and [WR]5-AuNPs were tested for their efficiency to deliver a small interfering RNA molecule (siRNA) in human cervix adenocarcinoma (HeLa) cells. Flow cytometry investigation revealed that the intracellular uptake of a fluorescence-labeled non-targeting siRNA (200 nM) was enhanced in the presence of [WR]5 and [WR]5-AuNPs by 2- and 3.8-fold when compared with that of siRNA alone after 24 h incubation. Comparative toxicity results showed that [WR]5 and [WR]5-AuNPs were less toxic in cells compared to other available carrier systems, such as Lipofectamine. |
| Databáze: | MEDLINE |
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