Optimization of ELFSE DNA sequencing with EOF counterflow and microfluidics.

Autor: Fahrenkopf MA; Department of Chemical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA., Mukherjee T, Ydstie BE, Schneider JW
Jazyk: angličtina
Zdroj: Electrophoresis [Electrophoresis] 2014 Dec; Vol. 35 (23), pp. 3408-14. Date of Electronic Publication: 2014 Oct 20.
DOI: 10.1002/elps.201400266
Abstrakt: We present a nonlinear optimization study of different implementations of the DNA electrophoretic method "End-labeled Free-solution Electrophoresis" in commercial capillary electrophoresis systems and microfluidics to improve the time required for readout. Here, the effect of electro-osmotic counterflows and snap-shot detection are considered to allow for detection of peaks soon after they are electorphoretically resolved. Using drag tags available in micelle form, we identify a design capable of sequencing 600 bases in 2.8 min.
(© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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