Oxytocin decreases colonic motility of cold water stressed rats via oxytocin receptors.

Autor:	Yang X; Xiao Yang, Tao-Fang Xi, Yu-Xian Li, Hai-Hong Wang, Ying Qin, Jie-Ping Zhang, Wen-Ting Cai, Meng-Ting Huang, Ji-Qiao Shen, Xi-Min Fan, Dong-Ping Xie, Department of Physiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200092, China., Xi TF; Xiao Yang, Tao-Fang Xi, Yu-Xian Li, Hai-Hong Wang, Ying Qin, Jie-Ping Zhang, Wen-Ting Cai, Meng-Ting Huang, Ji-Qiao Shen, Xi-Min Fan, Dong-Ping Xie, Department of Physiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200092, China., Li YX; Xiao Yang, Tao-Fang Xi, Yu-Xian Li, Hai-Hong Wang, Ying Qin, Jie-Ping Zhang, Wen-Ting Cai, Meng-Ting Huang, Ji-Qiao Shen, Xi-Min Fan, Dong-Ping Xie, Department of Physiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200092, China., Wang HH; Xiao Yang, Tao-Fang Xi, Yu-Xian Li, Hai-Hong Wang, Ying Qin, Jie-Ping Zhang, Wen-Ting Cai, Meng-Ting Huang, Ji-Qiao Shen, Xi-Min Fan, Dong-Ping Xie, Department of Physiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200092, China., Qin Y; Xiao Yang, Tao-Fang Xi, Yu-Xian Li, Hai-Hong Wang, Ying Qin, Jie-Ping Zhang, Wen-Ting Cai, Meng-Ting Huang, Ji-Qiao Shen, Xi-Min Fan, Dong-Ping Xie, Department of Physiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200092, China., Zhang JP; Xiao Yang, Tao-Fang Xi, Yu-Xian Li, Hai-Hong Wang, Ying Qin, Jie-Ping Zhang, Wen-Ting Cai, Meng-Ting Huang, Ji-Qiao Shen, Xi-Min Fan, Dong-Ping Xie, Department of Physiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200092, China., Cai WT; Xiao Yang, Tao-Fang Xi, Yu-Xian Li, Hai-Hong Wang, Ying Qin, Jie-Ping Zhang, Wen-Ting Cai, Meng-Ting Huang, Ji-Qiao Shen, Xi-Min Fan, Dong-Ping Xie, Department of Physiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200092, China., Huang MT; Xiao Yang, Tao-Fang Xi, Yu-Xian Li, Hai-Hong Wang, Ying Qin, Jie-Ping Zhang, Wen-Ting Cai, Meng-Ting Huang, Ji-Qiao Shen, Xi-Min Fan, Dong-Ping Xie, Department of Physiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200092, China., Shen JQ; Xiao Yang, Tao-Fang Xi, Yu-Xian Li, Hai-Hong Wang, Ying Qin, Jie-Ping Zhang, Wen-Ting Cai, Meng-Ting Huang, Ji-Qiao Shen, Xi-Min Fan, Dong-Ping Xie, Department of Physiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200092, China., Fan XM; Xiao Yang, Tao-Fang Xi, Yu-Xian Li, Hai-Hong Wang, Ying Qin, Jie-Ping Zhang, Wen-Ting Cai, Meng-Ting Huang, Ji-Qiao Shen, Xi-Min Fan, Dong-Ping Xie, Department of Physiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200092, China., Shi XZ; Xiao Yang, Tao-Fang Xi, Yu-Xian Li, Hai-Hong Wang, Ying Qin, Jie-Ping Zhang, Wen-Ting Cai, Meng-Ting Huang, Ji-Qiao Shen, Xi-Min Fan, Dong-Ping Xie, Department of Physiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200092, China., Xie DP; Xiao Yang, Tao-Fang Xi, Yu-Xian Li, Hai-Hong Wang, Ying Qin, Jie-Ping Zhang, Wen-Ting Cai, Meng-Ting Huang, Ji-Qiao Shen, Xi-Min Fan, Dong-Ping Xie, Department of Physiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200092, China.
Jazyk:	angličtina
Zdroj:	World journal of gastroenterology [World J Gastroenterol] 2014 Aug 21; Vol. 20 (31), pp. 10886-94.
DOI:	10.3748/wjg.v20.i31.10886
Abstrakt:	Aim: To investigate whether cold water intake into the stomach affects colonic motility and the involvement of the oxytocin-oxytocin receptor pathway in rats. Methods: Female Sprague Dawley rats were used and some of them were ovariectomized. The rats were subjected to gastric instillation with cold (0-4 °C, cold group) or room temperature (20-25 °C, control group) saline for 14 consecutive days. Colon transit was determined with a bead inserted into the colon. Colonic longitudinal muscle strips were prepared to investigate the response to oxytocin in vitro. Plasma concentration of oxytocin was detected by ELISA. Oxytocin receptor expression was investigated by Western blot analysis. Immunohistochemistry was used to locate oxytocin receptors. Results: Colon transit was slower in the cold group than in the control group (P < 0.05). Colonic smooth muscle contractile response to oxytocin decreased, and the inhibitory effect of oxytocin on muscle contractility was enhanced by cold water intake (0.69 ± 0.08 vs 0.88 ± 0.16, P < 0.05). Atosiban and tetrodotoxin inhibited the effect of oxytocin on colonic motility. Oxytocin receptors were located in the myenteric plexus, and their expression was up-regulated in the cold group (P < 0.05). Cold water intake increased blood concentration of oxytocin, but this effect was attenuated in ovariectomized rats (286.99 ± 83.72 pg/mL vs 100.56 ± 92.71 pg/mL, P < 0.05). However, in ovariectomized rats, estradiol treatment increased blood oxytocin, and the response of colonic muscle strips to oxytocin was attenuated. Conclusion: Cold water intake inhibits colonic motility partially through oxytocin-oxytocin receptor signaling in the myenteric nervous system pathway, which is estrogen dependent.
Databáze:	MEDLINE
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