Myocardial regeneration after implantation of porcine small intestinal submucosa in the left ventricle.

Autor:	Ramos CM; Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil., Francisco JC; Pontifícia Universidade Católica do Paraná (PUCPR), Universidade Federal do Paraná (UFPR), Curitiba, PR, Brazil., Curitiba, PR, Brazil., Olandoski M; Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil., Carvalho KA; Faculdade Pequeno Príncipe, Curitiba, PR, Brazil., Cunha R; Faculdade Pequeno Príncipe, Curitiba, PR, Brazil., Erbano BO; Faculdade Evangélica do Paraná, Curitiba, PR, Brazil., Jorge LF; Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil., Baena CP; Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil., Amaral VF; Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil., Noronha L; Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil., Macedo RM; Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil., Faria-Neto JR; Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil., Guarita-Souza LC; Pontifícia Universidade Católica do Paraná (PUCPR), Universidade Federal do Paraná (UFPR), Curitiba, PR, Brazil., Curitiba, PR, Brazil.
Jazyk:	angličtina
Zdroj:	Revista brasileira de cirurgia cardiovascular : orgao oficial da Sociedade Brasileira de Cirurgia Cardiovascular [Rev Bras Cir Cardiovasc] 2014 Apr-Jun; Vol. 29 (2), pp. 202-13.
DOI:	10.5935/1678-9741.20140070
Abstrakt:	Introduction: Most cardiomyocytes do not regenerate after myocardial infarction. Porcine small intestinal submucosa has been shown to be effective in tissue repair. Objective: To evaluate myocardial tissue regeneration and functional effects of SIS implantation in pigs after left ventriculotomy. Methods: Fifteen pigs were assigned to two groups: porcine small intestinal submucosa (SIS) (N=10) and control (N=5). The SIS group underwent a mini sternotomy, left ventriculotomy and placement of a SIS patch. The control group underwent a sham procedure. Echocardiography was performed before and 60 days after the surgical procedure. Histological analysis was performed with hematoxylin-eosin stain and markers for actin 1A4, anti sarcomeric actin, connexin43 and factor VIII. Results: Weight gain was similar in both groups. Echocardiography analysis revealed no difference between groups regarding end diastolic and systolic diameters and left ventricular ejection fraction, both pre (P=0.118, P=0.313, P=0.944) and post procedure (P=0.333, P=0.522, P=0.628). Both groups showed an increase in end diastolic (P<0,001 for both) and systolic diameter 60 days after surgery (P=0.005, SIS group and P=0.004, control group). New cardiomyocytes, blood vessels and inflammatory reactions were histologically identified in the SIS group. Conclusion: SIS implantation in pigs after left ventriculotomy was associated with angiomuscular regeneration and no damage in cardiac function. Competing Interests: STATEMENT The authors declare no conflicts of interest.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu