CCR2(+)CD103(-) intestinal dendritic cells develop from DC-committed precursors and induce interleukin-17 production by T cells.

Autor: Scott CL; 1] Institute of Infection, Immunity and Inflammation, College of Veterinary, Medical and Life Science, University of Glasgow, Glasgow, Scotland, UK [2] VIB Ghent University, Inflammation Research Centre (IRC), Laboratory of Immunoregulation, Ghent (Zwijnaarde), Belgium., Bain CC; Institute of Infection, Immunity and Inflammation, College of Veterinary, Medical and Life Science, University of Glasgow, Glasgow, Scotland, UK., Wright PB; Institute of Infection, Immunity and Inflammation, College of Veterinary, Medical and Life Science, University of Glasgow, Glasgow, Scotland, UK., Sichien D; VIB Ghent University, Inflammation Research Centre (IRC), Laboratory of Immunoregulation, Ghent (Zwijnaarde), Belgium., Kotarsky K; Immunology Section, Lund University, Lund, Sweden., Persson EK; Immunology Section, Lund University, Lund, Sweden., Luda K; Immunology Section, Lund University, Lund, Sweden., Guilliams M; VIB Ghent University, Inflammation Research Centre (IRC), Laboratory of Immunoregulation, Ghent (Zwijnaarde), Belgium., Lambrecht BN; VIB Ghent University, Inflammation Research Centre (IRC), Laboratory of Immunoregulation, Ghent (Zwijnaarde), Belgium., Agace WW; 1] Immunology Section, Lund University, Lund, Sweden [2] Department of International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark., Milling SW; Institute of Infection, Immunity and Inflammation, College of Veterinary, Medical and Life Science, University of Glasgow, Glasgow, Scotland, UK., Mowat AM; Institute of Infection, Immunity and Inflammation, College of Veterinary, Medical and Life Science, University of Glasgow, Glasgow, Scotland, UK.
Jazyk: angličtina
Zdroj: Mucosal immunology [Mucosal Immunol] 2015 Mar; Vol. 8 (2), pp. 327-39. Date of Electronic Publication: 2014 Aug 20.
DOI: 10.1038/mi.2014.70
Abstrakt: The identification of intestinal macrophages (mφs) and dendritic cells (DCs) is a matter of intense debate. Although CD103(+) mononuclear phagocytes (MPs) appear to be genuine DCs, the nature and origins of CD103(-) MPs remain controversial. We show here that intestinal CD103(-)CD11b(+) MPs can be separated clearly into DCs and mφs based on phenotype, gene profile, and kinetics. CD64(-)CD103(-)CD11b(+) MPs are classical DCs, being derived from Flt3 ligand-dependent, DC-committed precursors, not Ly6C(hi) monocytes. Surprisingly, a significant proportion of these CD103(-)CD11b(+) DCs express CCR2 and there is a selective decrease in CD103(-)CD11b(+) DCs in mice lacking this chemokine receptor. CCR2(+)CD103(-) DCs are present in both the murine and human intestine, drive interleukin (IL)-17a production by T cells in vitro, and show constitutive expression of IL-12/IL-23p40. These data highlight the heterogeneity of intestinal DCs and reveal a bona fide population of CCR2(+) DCs that is involved in priming mucosal T helper type 17 (Th17) responses.
Databáze: MEDLINE
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