Global scaling down of excitatory postsynaptic responses in cerebellar Purkinje cells impairs developmental synapse elimination.

Autor:	Kawata S; Department of Neurophysiology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan., Miyazaki T; Department of Anatomy, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan., Yamazaki M; Department of Cellular Neurobiology, Brain Research Institute, Niigata University, Niigata 951-8585, Japan., Mikuni T; Department of Neurophysiology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan., Yamasaki M; Department of Anatomy, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan., Hashimoto K; Department of Neurophysiology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; Department of Neurophysiology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8551, Japan., Watanabe M; Department of Anatomy, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan., Sakimura K; Department of Cellular Neurobiology, Brain Research Institute, Niigata University, Niigata 951-8585, Japan., Kano M; Department of Neurophysiology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. Electronic address: mkano-tky@m.u-tokyo.ac.jp.
Jazyk:	angličtina
Zdroj:	Cell reports [Cell Rep] 2014 Aug 21; Vol. 8 (4), pp. 1119-29. Date of Electronic Publication: 2014 Aug 07.
DOI:	10.1016/j.celrep.2014.07.014
Abstrakt:	Synapse elimination is crucial for precise neural circuit formation during postnatal development. We examined how relative differences in synaptic strengths among competing inputs and/or absolute synaptic strengths contribute to climbing fiber (CF) to Purkinje cell (PC) synapse elimination in the cerebellum. We generated mice with PC-selective deletion of stargazin (TARP γ-2), the major AMPA receptor auxiliary subunit in PCs (γ-2 PC-KO mice). Whereas relative differences between "strong" and "weak" CF-mediated postsynaptic response are preserved, absolute strengths of CF inputs are scaled down globally in PCs of γ-2 PC-KO mice. Although the early phase of CF elimination is normal, dendritic translocation of the strongest CF and the late phase of CF elimination that requires Ca(2+)-dependent activation of Arc/Arg3.1 in PCs are both impaired in γ-2 PC-KO mice. We conclude that, although relative differences in CF synaptic inputs are initially essential, proper synaptic scaling is crucial for accomplishing CF synapse elimination. (Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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