Synthetic biology. Programmable on-chip DNA compartments as artificial cells.
| Autor: | Karzbrun E; Department of Materials and Interfaces, Weizmann Institute of Science, Rehovot 76100, Israel., Tayar AM; Department of Materials and Interfaces, Weizmann Institute of Science, Rehovot 76100, Israel., Noireaux V; Department of Physics, University of Minnesota, Minneapolis, MN 55455, USA., Bar-Ziv RH; Department of Materials and Interfaces, Weizmann Institute of Science, Rehovot 76100, Israel. roy.bar-ziv@weizmann.ac.il. |
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| Jazyk: | angličtina |
| Zdroj: | Science (New York, N.Y.) [Science] 2014 Aug 15; Vol. 345 (6198), pp. 829-32. |
| DOI: | 10.1126/science.1255550 |
| Abstrakt: | The assembly of artificial cells capable of executing synthetic DNA programs has been an important goal for basic research and biotechnology. We assembled two-dimensional DNA compartments fabricated in silicon as artificial cells capable of metabolism, programmable protein synthesis, and communication. Metabolism is maintained by continuous diffusion of nutrients and products through a thin capillary, connecting protein synthesis in the DNA compartment with the environment. We programmed protein expression cycles, autoregulated protein levels, and a signaling expression gradient, equivalent to a morphogen, in an array of interconnected compartments at the scale of an embryo. Gene expression in the DNA compartment reveals a rich, dynamic system that is controlled by geometry, offering a means for studying biological networks outside a living cell. (Copyright © 2014, American Association for the Advancement of Science.) |
| Databáze: | MEDLINE |
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