Kinetics of recA and recX induction in drug-susceptible and MDR clinical strains of Mycobacterium tuberculosis.
| Autor: | Bhatter P; The Foundation for Medical Research, 84-A, R. G. Thadani Marg, Worli, Mumbai 400018, India., Chatterjee A; The Foundation for Medical Research, 84-A, R. G. Thadani Marg, Worli, Mumbai 400018, India., Mistry N; The Foundation for Medical Research, 84-A, R. G. Thadani Marg, Worli, Mumbai 400018, India fmr@fmrindia.org. |
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| Jazyk: | angličtina |
| Zdroj: | The Journal of antimicrobial chemotherapy [J Antimicrob Chemother] 2014 Dec; Vol. 69 (12), pp. 3199-202. Date of Electronic Publication: 2014 Aug 11. |
| DOI: | 10.1093/jac/dku319 |
| Abstrakt: | Objectives: To investigate and compare the expression of recA and recX, components of the SOS pathway, following rifampicin treatment in drug-susceptible and MDR clinical strains of Mycobacterium tuberculosis. Methods: Strains (M. tuberculosis and Mycobacterium smegmatis) were subjected to rifampicin- and mitomycin-induced stress for 36 h followed by RNA extraction. recA and recX in the RNA extract were estimated using qRT-PCR. Results: The MDR clinical strain induced faster (24 h) and higher (7-fold) levels of recA as compared with the drug-susceptible strain (36 h) in response to rifampicin. recX levels were found to rise with an increase in levels of recA; however, the levels were relatively higher than recA. Conclusions: Drug-susceptible and MDR strains have different kinetics of induction of DNA repair. (© The Author 2014. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.) |
| Databáze: | MEDLINE |
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