| Autor: |
Cho BO; a Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute , Jeongeup , Republic of Korea., So Y, Jin CH, Nam BM, Yee ST, Jeong IY |
| Jazyk: |
angličtina |
| Zdroj: |
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2014; Vol. 78 (12), pp. 2051-8. Date of Electronic Publication: 2014 Aug 08. |
| DOI: |
10.1080/09168451.2014.948377 |
| Abstrakt: |
3-deoxysilybin (3-DS), also known as (-)-isosilandrin A, is a natural flavonoid of Silybum marianum. This study was designed to investigate the anti-inflammatory effect and the underlying molecular mechanisms of 3-DS in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. 3-DS dose-dependently inhibited the production of NO and the expression of iNOS in LPS-stimulated RAW264.7 macrophages. 3-DS also inhibited the production of pro-inflammatory cytokines (MCP-1, TNF-α, IL-6, and IL-1β) in LPS-stimulated RAW264.7 macrophages. Moreover, 3-DS decreased the NF-κB DNA binding activity in LPS-stimulated RAW264.7 macrophages. Furthermore, 3-DS suppressed NF-κB activation by inhibiting the degradation of IκBα and nuclear translocation of p65 subunit of NF-κB in LPS-stimulated RAW264.7 macrophages. Taken together, the present study suggests for the first time that 3-DS may exhibit an anti-inflammatory effect through the suppression of NF-κB transcriptional activation in LPS-stimulated RAW264.7 macrophages. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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