| Autor: |
Prando TB; Instituto de Ciências Biológicas, Médicas e da Saúde, Universidade Paranaense, P.O. Box 224, 87502-210 Umuarama, PR, Brazil., Baciquete Tda F; Instituto de Ciências Biológicas, Médicas e da Saúde, Universidade Paranaense, P.O. Box 224, 87502-210 Umuarama, PR, Brazil., Vieira JA; Instituto de Ciências Biológicas, Médicas e da Saúde, Universidade Paranaense, P.O. Box 224, 87502-210 Umuarama, PR, Brazil., Bressan J; Instituto de Ciências Biológicas, Médicas e da Saúde, Universidade Paranaense, P.O. Box 224, 87502-210 Umuarama, PR, Brazil., Gasparotto FM; Laboratório de Farmacologia Cardiovascular, Faculdade de Ciências da Saúde, Universidade Federal da Grande Dourados, Rodovia Dourados, Itahum, km 12, P.O. Box 533, 79.804-970 Dourados, MS, Brazil., Jesus DR; Instituto de Ciências Biológicas, Médicas e da Saúde, Universidade Paranaense, P.O. Box 224, 87502-210 Umuarama, PR, Brazil., Cardozo Junior EL; Instituto de Ciências Biológicas, Médicas e da Saúde, Universidade Paranaense, P.O. Box 224, 87502-210 Umuarama, PR, Brazil., Lourenço EL; Instituto de Ciências Biológicas, Médicas e da Saúde, Universidade Paranaense, P.O. Box 224, 87502-210 Umuarama, PR, Brazil., Gasparotto Junior A; Laboratório de Farmacologia Cardiovascular, Faculdade de Ciências da Saúde, Universidade Federal da Grande Dourados, Rodovia Dourados, Itahum, km 12, P.O. Box 533, 79.804-970 Dourados, MS, Brazil. |
| Abstrakt: |
Several species of the genus Piper are known in Brazilian folk medicine as having diuretic activity. So, we propose to investigate the acute diuretic activity and the possible toxic effects of Piper glabratum Kunth, popularly known as false Jaborandi. Additionally, we propose to check whether there is any correlation between the biological activities of the crude extract (MEPG) and its 2-methoxy-4,5-methylenedioxy-trans-cinnamoyl-pyrrolidine (MMCP) in Wistar rats. The MEPG was fractioned by chromatography column and the MMCP was identified by analyses of (1)H and (13)C RMN spectral data and correlations. Both MEPG and MMCP were assayed for diuretic activity. The preparations obtained were orally administered in a single dose to rats. The urine excretion, pH, density, conductivity, and content of Na(+), K(+), Cl(-), and HCO3 (-) were measured in the urine of saline-loaded animals. Additionally, acute toxicity of the extract was also evaluated. MMCP at doses of 30 mg/kg was able to increase the urine volume, pH, and HCO3 (-) excretion. Moreover, high dosage of MEPG showed important liver toxicity and elevated mortality when injected intraperitoneally. The results indicate that the MMCP shows important diuretic properties when administered in Wistar rats. Additionally, MEPG can induce important acute toxicity if given in high doses. |
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