Age-related sperm DNA methylation changes are transmitted to offspring and associated with abnormal behavior and dysregulated gene expression.

Autor: Milekic MH; Department of Psychiatry, Columbia University and The New York State Psychiatric Institute, New York, NY, USA., Xin Y; Department of Psychiatry, Columbia University and The New York State Psychiatric Institute, New York, NY, USA., O'Donnell A; Department of Genetics and Development, Columbia University, New York, NY, USA., Kumar KK; Department of Psychiatry, Columbia University and The New York State Psychiatric Institute, New York, NY, USA., Bradley-Moore M; Department of Psychiatry, Columbia University and The New York State Psychiatric Institute, New York, NY, USA., Malaspina D; 1] Department of Psychiatry, New York University, New York, NY, USA [2] Department of Psychiatry, New York University, and The NY OMH Creedmoor Psychiatric Center, New York, NY, USA., Moore H; Department of Psychiatry, Columbia University and The New York State Psychiatric Institute, New York, NY, USA., Brunner D; 1] Department of Psychiatry, Columbia University and The New York State Psychiatric Institute, New York, NY, USA [2] PsychoGenics, New York, NY, USA., Ge Y; Department of Neurology, Mount Sinai School of Medicine, New York, NY, USA., Edwards J; Center for Pharmacogenomics, Department of Medicine, Washington University School of Medicine, St Louis, MO, USA., Paul S; Helen & Robert Appel Institute for Alzheimer's Research, Mind and Brain Institute, Weill Cornell Medical School, New York, NY, USA., Haghighi FG; Department of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Gingrich JA; Department of Psychiatry, Columbia University and The New York State Psychiatric Institute, New York, NY, USA.
Jazyk: angličtina
Zdroj: Molecular psychiatry [Mol Psychiatry] 2015 Aug; Vol. 20 (8), pp. 995-1001. Date of Electronic Publication: 2014 Aug 05.
DOI: 10.1038/mp.2014.84
Abstrakt: Advanced paternal age (APA) has been shown to be a significant risk factor in the offspring for neurodevelopmental psychiatric disorders, such as schizophrenia and autism spectrum disorders. During aging, de novo mutations accumulate in the male germline and are frequently transmitted to the offspring with deleterious effects. In addition, DNA methylation during spermatogenesis is an active process, which is susceptible to errors that can be propagated to subsequent generations. Here we test the hypothesis that the integrity of germline DNA methylation is compromised during the aging process. A genome-wide DNA methylation screen comparing sperm from young and old mice revealed a significant loss of methylation in the older mice in regions associated with transcriptional regulation. The offspring of older fathers had reduced exploratory and startle behaviors and exhibited similar brain DNA methylation abnormalities as observed in the paternal sperm. Offspring from old fathers also had transcriptional dysregulation of developmental genes implicated in autism and schizophrenia. Our findings demonstrate that DNA methylation abnormalities arising in the sperm of old fathers are a plausible mechanism to explain some of the risks that APA poses to resulting offspring.
Databáze: MEDLINE