| Autor: |
Premalatha R; Department of Biochemistry & Molecular Biology, Pondicherry University, Pondicherry, 605014, India, prema.srikumar@gmail.com., Srikumar K, Vijayalaksmi D, Kumar GN, Mathur PP |
| Jazyk: |
angličtina |
| Zdroj: |
Molecular biology reports [Mol Biol Rep] 2014 Nov; Vol. 41 (11), pp. 7447-61. Date of Electronic Publication: 2014 Aug 05. |
| DOI: |
10.1007/s11033-014-3632-5 |
| Abstrakt: |
Cholesterol is the template for steroid hormone biosynthesis. Cholesterol homeostasis is regulated by Cyt-P450 oxygenated cholesterols acting as ligands on LXR-α and LXR-β transcription factors that are now emerging as drug targets. Heterodimerization of LXRs with retinoic acid receptor is considered a prerequisite for target gene activation. Dietary plant oxysterol 28-homobrassinolide (28-HB) is a proven antihyperglycemic and a pro-steroidogenic agent in the rat. Whether 28-HB has a role in LXR gene expression was therefore investigated using oral gavage (15 days) of 28-HB (333 µg/kg b w) to normal and diabetic rat. PCR amplified LXR-α and β mRNA transcripts from treated rat liver and testis exhibited quantitative differences in their expression. Conformational differences in 28-HB docking to LXR-α and β binding domains were also noted through in silico studies, LXR-β adopting lesser specificity. We report that 28-HB transactivates LXR genes in the rat tissues. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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