Identification of Corynebacterium spp. isolated from bovine intramammary infections by matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Autor: Gonçalves JL; Department of Animal Science, School of Veterinary Medicine and Animal Science (USP), Pirassununga, SP, Brazil., Tomazi T; Department of Animal Science, School of Veterinary Medicine and Animal Science (USP), Pirassununga, SP, Brazil., Barreiro JR; Department of Animal Science, School of Veterinary Medicine and Animal Science (USP), Pirassununga, SP, Brazil., Braga PA; ThoMSon MS Laboratory, Institute of Chemistry, University of Campinas (UNICAMP), Campinas, SP, Brazil., Ferreira CR; ThoMSon MS Laboratory, Institute of Chemistry, University of Campinas (UNICAMP), Campinas, SP, Brazil., Araújo Junior JP; Institute of Biosciences, State University of São Paulo 'Júlio de Mesquita' (UNESP), Botucatu, SP, Brazil., Eberlin MN; ThoMSon MS Laboratory, Institute of Chemistry, University of Campinas (UNICAMP), Campinas, SP, Brazil., dos Santos MV; Department of Animal Science, School of Veterinary Medicine and Animal Science (USP), Pirassununga, SP, Brazil. Electronic address: mveiga@usp.br.
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2014 Sep 17; Vol. 173 (1-2), pp. 147-51. Date of Electronic Publication: 2014 Jul 07.
DOI: 10.1016/j.vetmic.2014.06.028
Abstrakt: Corynebacterium species (spp.) are among the most frequently isolated pathogens associated with subclinical mastitis in dairy cows. However, simple, fast, and reliable methods for the identification of species of the genus Corynebacterium are not currently available. This study aimed to evaluate the usefulness of matrix-assisted laser desorption ionization/mass spectrometry (MALDI-TOF MS) for identifying Corynebacterium spp. isolated from the mammary glands of dairy cows. Corynebacterium spp. were isolated from milk samples via microbiological culture (n=180) and were analyzed by MALDI-TOF MS and 16S rRNA gene sequencing. Using MALDI-TOF MS methodology, 161 Corynebacterium spp. isolates (89.4%) were correctly identified at the species level, whereas 12 isolates (6.7%) were identified at the genus level. Most isolates that were identified at the species level with 16 S rRNA gene sequencing were identified as Corynebacterium bovis (n=156; 86.7%) were also identified as C. bovis with MALDI-TOF MS. Five Corynebacterium spp. isolates (2.8%) were not correctly identified at the species level with MALDI-TOF MS and 2 isolates (1.1%) were considered unidentified because despite having MALDI-TOF MS scores >2, only the genus level was correctly identified. Therefore, MALDI-TOF MS could serve as an alternative method for species-level diagnoses of bovine intramammary infections caused by Corynebacterium spp.
(Copyright © 2014 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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