Serological evidence for Saint Louis encephalitis virus in free-ranging New World monkeys and horses within the upper Paraná River basin region, Southern Brazil.

Autor:	Svoboda WK; Instituto Latino-Americano de Ciências da Vida e da Natureza (ILACVN), Universidade Federal da Integração Latino-Americana (UNILA), Foz do Iguaçu, PR, Brasil., Martins LC; Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ananindeua, PA, Brazil., Malanski Lde S; Instituto Chico Mendes de Conservação da Biodiversidade, Porto Velho, RO, Brasil., Shiozawa MM; Escola de Medicina Veterinária, Universidade Norte do Paraná, Arapongas, PR, Brasil., Spohr KA; Escola de Medicina Veterinária, Universidade de Cuiabá, Cuiabá, MT., Hilst CL; Departamento de Clínica Veterinária, Universidade Estadual de Londrina, Londrina, PR, Basil., Aguiar LM; Universidade Federal da Integração Latino-Americana, Foz do Iguaçu, PR., Ludwig G; Centro Nacional de Pesquisa e Conservação de Primatas Brasileiros, Instituto Chico Mendes de Conservação da Biodiversidade, João Pessoa, PB., Passos Fde C; Departamento de Zoologia, Universidade Federal do Paraná, Curitiba, PR, Brasil., Silva LR; Curitiba, Curitiba, PR, Brasil., Headley SA; Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Londrina, PR, Basil., Navarro IT; Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Londrina, PR, Basil.
Jazyk:	angličtina
Zdroj:	Revista da Sociedade Brasileira de Medicina Tropical [Rev Soc Bras Med Trop] 2014 May-Jun; Vol. 47 (3), pp. 280-6.
DOI:	10.1590/0037-8682-0083-2014
Abstrakt:	Introduction: Saint Louis encephalitis virus (SLEV) primarily occurs in the Americas and produces disease predominantly in humans. This study investigated the serological presence of SLEV in nonhuman primates and horses from southern Brazil. Methods: From June 2004 to December 2005, sera from 133 monkeys (Alouatta caraya, n=43; Sapajus nigritus, n=64; Sapajus cay, n=26) trap-captured at the Paraná River basin region and 23 blood samples from farm horses were obtained and used for the serological detection of a panel of 19 arboviruses. All samples were analyzed in a hemagglutination inhibition (HI) assay; positive monkey samples were confirmed in a mouse neutralization test (MNT). Additionally, all blood samples were inoculated into C6/36 cell culture for viral isolation. Results: Positive seroreactivity was only observed for SLEV. A prevalence of SLEV antibodies in sera was detected in Alouatta caraya (11.6%; 5/43), Sapajus nigritus (12.5%; 8/64), and S. cay (30.8%; 8/26) monkeys with the HI assay. Of the monkeys, 2.3% (1/42) of A. caraya, 6.3% 94/64) of S. nigritus, and 15.4% (4/26) of S. cay were positive for SLEV in the MNT. Additionally, SLEV antibodies were detected by HI in 39.1% (9/23) of the horses evaluated in this study. Arboviruses were not isolated from any blood sample. Conclusions: These results confirmed the presence of SLEV in nonhuman primates and horses from southern Brazil. These findings most likely represent the first detection of this virus in nonhuman primates beyond the Amazon region. The detection of SLEV in animals within a geographical region distant from the Amazon basin suggests that there may be widespread and undiagnosed dissemination of this disease in Brazil.
Databáze:	MEDLINE
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