Sensitive impedimetric biosensor for direct detection of diazinon based on lipases.

Autor:	Zehani N; UMR 5280, Institut des Sciences Analytiques, Université de Lyon Villeurbanne, France ; Laboratory of Environmental Engineering, Faculty of Engineering Sciences, University of Annaba Annaba, Algeria., Dzyadevych SV; Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine Kiev, Ukraine., Kherrat R; Laboratory of Environmental Engineering, Faculty of Engineering Sciences, University of Annaba Annaba, Algeria., Jaffrezic-Renault NJ; UMR 5280, Institut des Sciences Analytiques, Université de Lyon Villeurbanne, France.
Jazyk:	angličtina
Zdroj:	Frontiers in chemistry [Front Chem] 2014 Jul 04; Vol. 2, pp. 44. Date of Electronic Publication: 2014 Jul 04 (Print Publication: 2014).
DOI:	10.3389/fchem.2014.00044
Abstrakt:	Two novel impedimetric biosensors for highly sensitive and rapid quantitative detection of diazinon in aqueous medium were developed using two types of lipase, from Candida Rugosa (microbial source) (CRL) and from porcine pancreas (animal source) (PPL) immobilized on functionalized gold electrode. Lipase is characterized to specifically catalyze the hydrolysis of ester functions leading to the transformation of diazinon into diethyl phosphorothioic acid (DETP) and 2-isopropyl-4-methyl-6-hydroxypyrimidine (IMHP). The developed biosensors both presented a wide range of linearity up to 50 μM with a detection limit of 10 nM for Candida Rugosa biosensor and 0.1 μM for porcine pancreas biosensor. A comparative study was carried out between the two biosensors and results showed higher efficiency of Candida Rugosa sensor. Moreover, it presented good accuracy and reproducibility, had very good storage and multiple use stability for 25 days when stored at 4°C.
Databáze:	MEDLINE
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