| Autor: |
Belova OV; Research Institute of Physico-Chemical Medicine, Federal Biomedical Agency of Russia, Moscow, Russia, olgabelova49@yandex.ru., Sergienko VI, Arion VY, Lukanidina TA, Moskvina SN, Zimina IV, Borisenko GG, Lutsenko GV, Grechikhina MV, Kovaleva EV, Klyuchnikova ZhI |
| Jazyk: |
angličtina |
| Zdroj: |
Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2014 Jul; Vol. 157 (3), pp. 371-4. Date of Electronic Publication: 2014 Jul 30. |
| DOI: |
10.1007/s10517-014-2569-y |
| Abstrakt: |
Subfraction with a molecular weight >250 kDa isolated from porcine skin and inhibiting the proliferation of A431 human carcinoma epidermoid cells was purified by DEAE 32 anion exchange chromatography with NaCl concentration step-gradient. The effects of the initial subfraction and fractions obtained by separation in DEAE 32 on the proliferation of A431 human carcinoma epidermoid cells were studied in vitro in two tests (MTT and fluorescent test). The more sensitive fluorescent test showed the highest inhibitory activity of fraction No. 2 released from the column at 0.15 M NaCl. One major protein component and a series of minor protein components were detected in this fraction by vertical PAAG-SDS electrophoresis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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