Archaeal and bacterial diversity in five different hydrothermal ponds in the Copahue region in Argentina.

Autor: Sofía Urbieta M; CINDEFI (CCT La Plata-CONICET, UNLP), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47 y 115, 1900 La Plata, Argentina. Electronic address: msurbieta@biol.unlp.edu.ar., Toril EG; Centro de Astrobiología (INTA-CSIC), Instituto Nacional de Técnica Aeroespacial, Carretera de Ajalvir Km. 4, 28850, Torrejón de Ardoz, Madrid, Spain., Alejandra Giaveno M; IDEPA (CCT Comahue-CONICET, UNCo), Dpto. Química, Facultad de Ingeniería, Universidad Nacional del Comahue, Buenos Aires 1400, 8300 Neuquén, Argentina., Bazán AA; Centro de Astrobiología (INTA-CSIC), Instituto Nacional de Técnica Aeroespacial, Carretera de Ajalvir Km. 4, 28850, Torrejón de Ardoz, Madrid, Spain., Donati ER; CINDEFI (CCT La Plata-CONICET, UNLP), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47 y 115, 1900 La Plata, Argentina.
Jazyk: angličtina
Zdroj: Systematic and applied microbiology [Syst Appl Microbiol] 2014 Sep; Vol. 37 (6), pp. 429-41. Date of Electronic Publication: 2014 Jul 14.
DOI: 10.1016/j.syapm.2014.05.012
Abstrakt: Copahue is an acidic geothermal volcanic region in the northwest corner of Neuquén Province, Argentina. In the area, there are various ponds, pools and hot springs with different temperatures, pH values and levels of anthropogenic influence. In this study, the prokaryotic biodiversity of five representative ponds was studied by using two complementary molecular ecology techniques: phylogenetic analysis of 16S rRNA bacterial and archaeal genes and FISH (or CARD-FISH) for quantitative estimation of biodiversity. The results, supported by multivariate statistical analysis, showed that the biodiversity in Copahue ponds seemed to be determined by temperature. High temperature ponds were dominated by archaea, mainly apparently novel representatives from the orders Sulfolobales and Thermoplasmatales that had no close cultivated relatives. By contrast, moderate temperature ponds were colonised by well-characterised sulphur-oxidising bacteria related to acidic environments, such as other geothermal sites or acid mine drainage, and archaea were absent. By combining the biodiversity results from this study and the reported physicochemical features of Copahue, a preliminary model of the possible biogeochemical interaction was outlined for moderate and high temperature ponds.
(Copyright © 2014 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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