Delta-sleep inducing peptide entrapment in the charged macroporous matrices.

Autor: Sukhanova TV; Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Laboratory of Cell Interactions, Miklukho-Maklaya st., 16/10 Moscow, Russia. Electronic address: sukhanovat@mail.ru., Artyukhov AA; Mendeleyev University of Chemical Technology of Russia, Research and Teaching Center 'Biomaterials', Miusskaya sq., 9 Moscow, Russia., Gurevich YM; Mendeleyev University of Chemical Technology of Russia, Research and Teaching Center 'Biomaterials', Miusskaya sq., 9 Moscow, Russia., Semenikhina MA; Mendeleyev University of Chemical Technology of Russia, Research and Teaching Center 'Biomaterials', Miusskaya sq., 9 Moscow, Russia., Prudchenko IA; Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Laboratory of Peptide Chemistry, Miklukho-Maklaya st., 16/10 Moscow, Russia., Shtilman MI; Mendeleyev University of Chemical Technology of Russia, Research and Teaching Center 'Biomaterials', Miusskaya sq., 9 Moscow, Russia., Markvicheva EA; Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Laboratory Polymers for Biology, Miklukho-Maklaya st., 16/10 Moscow, Russia.
Jazyk: angličtina
Zdroj: Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2014 Sep; Vol. 42, pp. 461-5. Date of Electronic Publication: 2014 Jun 06.
DOI: 10.1016/j.msec.2014.05.059
Abstrakt: Various biomolecules, for example proteins, peptides etc., entrapped in polymer matrices, impact interactions between matrix and cells, including stimulation of cell adhesion and proliferation. Delta-sleep inducing peptide (DSIP) possesses numerous beneficial properties, including its abilities in burn treatment and neuronal protection. DSIP entrapment in two macroporous polymer matrices based on copolymer of dimethylaminoethyl methacrylate and methylen-bis-acrylamide (Co-DMAEMA-MBAA) and copolymer of acrylic acid and methylen-bis-acrylamide (Co-AA-MBAA) has been studied. Quite 100% of DSIP has been entrapped into positively charged Co-DMAEMA-MBAA matrix, while the quantity of DSIP adsorbed on negatively charged Co-AA-MBAA was only 2-6%. DSIP release from Co-DMAEMA-MBAA was observed in saline solutions (0.9% NaCl and PBS) while there was no DSIP release in water or 25% ethanol, thus ionic strength was a reason of this process.
(Copyright © 2014 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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