Laser ablation-inductively coupled plasma mass spectrometry: an emerging technology for detecting rare cells in tissue sections.

Autor:	Managh AJ; Centre for Analytical Science, Department of Chemistry, Loughborough University, Loughborough, Leicestershire LE11 3TU, United Kingdom;, Hutchinson RW; Electro Scientific Industries, Huntingdon, Cambridgeshire PE29 6XS, United Kingdom;, Riquelme P; Division of Experimental Surgery, Department of Surgery, University Hospital Regensburg, Regensburg 93053, Germany;, Broichhausen C; Division of Experimental Surgery, Department of Surgery, University Hospital Regensburg, Regensburg 93053, Germany;, Wege AK; Department of Obstetrics and Gynaecology, University Hospital Regensburg, Regensburg 93053, Germany;, Ritter U; Institute for Immunology, University of Regensburg, Regensburg 93053, Germany; and., Ahrens N; Department of Transfusion Medicine, University Hospital Regensburg, Regensburg 93053, Germany., Koehl GE; Division of Experimental Surgery, Department of Surgery, University Hospital Regensburg, Regensburg 93053, Germany;, Walter L; Division of Experimental Surgery, Department of Surgery, University Hospital Regensburg, Regensburg 93053, Germany;, Florian C; Division of Experimental Surgery, Department of Surgery, University Hospital Regensburg, Regensburg 93053, Germany;, Schlitt HJ; Division of Experimental Surgery, Department of Surgery, University Hospital Regensburg, Regensburg 93053, Germany;, Reid HJ; Centre for Analytical Science, Department of Chemistry, Loughborough University, Loughborough, Leicestershire LE11 3TU, United Kingdom;, Geissler EK; Division of Experimental Surgery, Department of Surgery, University Hospital Regensburg, Regensburg 93053, Germany;, Sharp BL; Centre for Analytical Science, Department of Chemistry, Loughborough University, Loughborough, Leicestershire LE11 3TU, United Kingdom;, Hutchinson JA; Division of Experimental Surgery, Department of Surgery, University Hospital Regensburg, Regensburg 93053, Germany; james.hutchinson@ukr.de.
Jazyk:	angličtina
Zdroj:	Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2014 Sep 01; Vol. 193 (5), pp. 2600-8. Date of Electronic Publication: 2014 Jul 23.
DOI:	10.4049/jimmunol.1400869
Abstrakt:	Administering immunoregulatory cells to patients as medicinal agents is a potentially revolutionary approach to the treatment of immunologically mediated diseases. Presently, there are no satisfactory, clinically applicable methods of tracking human cells in patients with adequate spatial resolution and target cell specificity over a sufficient period of time. Laser ablation-inductively coupled plasma mass spectrometry (LA-ICP-MS) represents a potential solution to the problem of detecting very rare cells in tissues. In this article, this exquisitely sensitive technique is applied to the tracking of gold-labeled human regulatory macrophages (Mregs) in immunodeficient mice. Optimal conditions for labeling Mregs with 50-nm gold particles were investigated by exposing Mregs in culture to variable concentrations of label: Mregs incubated with 3.5 × 10(9) particles/ml for 1 h incorporated an average of 3.39 × 10(8) Au atoms/cell without loss of cell viability. Analysis of single, gold-labeled Mregs by LA-ICP-MS registered an average of 1.9 × 10(5) counts/cell. Under these conditions, 100% labeling efficiency was achieved, and label was retained by Mregs for ≥36 h. Gold-labeled Mregs adhered to glass surfaces; after 24 h of culture, it was possible to colabel these cells with human-specific (154)Sm-tagged anti-HLA-DR or (174)Yb-tagged anti-CD45 mAbs. Following injection into immunodeficient mice, signals from gold-labeled human Mregs could be detected in mouse lung, liver, and spleen for at least 7 d by solution-based inductively coupled plasma mass spectrometry and LA-ICP-MS. These promising results indicate that LA-ICP-MS tissue imaging has great potential as an analytical technique in immunology. (Copyright © 2014 by The American Association of Immunologists, Inc.)
Databáze:	MEDLINE
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