[VNTR-genotyping of Vibrio cholerae strains isolated from objects in the territory of Russian Federation in 2012].
Autor: | Vodop'ianov AS, Mazrukho AB, Vodop'ianov SO, Mishan'kin BN, Kruglikov VD, Apkhangel'skaia IV, Oleĭnikov IP, Zubkova DA, Monakhova EV, Grigorenko LV |
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Jazyk: | ruština |
Zdroj: | Zhurnal mikrobiologii, epidemiologii i immunobiologii [Zh Mikrobiol Epidemiol Immunobiol] 2014 Mar-Apr (2), pp. 46-51. |
Abstrakt: | Aim: VNTR-typing of Vibrio cholerae strains isolated in the territory of Russian Federation in 2012. Materials and Methods: 71 Vibrio cholerae O3 and 3 V cholerae O1/O139 strains were used in the study. Genotyping was performed by using PCR for 5 VNTR-loci. Results: Multilocus VNTR-typing allowed to group the strains into 31 VNTR-genotypes. Genotypes were divided among 10 discrete clusters by results of a cluster analysis. The presence of tcpA gene is clearly linked with the presence of VcB locus. Each geographic region was characterized by their own VNTR-genotypes. Conclusion: In the course of the carried out VNTR-genotyping of V. cholerae isolated in 2012, 2 types of vibrio population formation were detected. A geographic attachment to specific regions was characteristic for most of the genotypes. |
Databáze: | MEDLINE |
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