[VNTR-genotyping of Vibrio cholerae strains isolated from objects in the territory of Russian Federation in 2012].

Autor: Vodop'ianov AS, Mazrukho AB, Vodop'ianov SO, Mishan'kin BN, Kruglikov VD, Apkhangel'skaia IV, Oleĭnikov IP, Zubkova DA, Monakhova EV, Grigorenko LV
Jazyk: ruština
Zdroj: Zhurnal mikrobiologii, epidemiologii i immunobiologii [Zh Mikrobiol Epidemiol Immunobiol] 2014 Mar-Apr (2), pp. 46-51.
Abstrakt: Aim: VNTR-typing of Vibrio cholerae strains isolated in the territory of Russian Federation in 2012.
Materials and Methods: 71 Vibrio cholerae O3 and 3 V cholerae O1/O139 strains were used in the study. Genotyping was performed by using PCR for 5 VNTR-loci.
Results: Multilocus VNTR-typing allowed to group the strains into 31 VNTR-genotypes. Genotypes were divided among 10 discrete clusters by results of a cluster analysis. The presence of tcpA gene is clearly linked with the presence of VcB locus. Each geographic region was characterized by their own VNTR-genotypes.
Conclusion: In the course of the carried out VNTR-genotyping of V. cholerae isolated in 2012, 2 types of vibrio population formation were detected. A geographic attachment to specific regions was characteristic for most of the genotypes.
Databáze: MEDLINE