Intracellular oxidation of hydroethidine: compartmentalization and cytotoxicity of oxidation products.

Autor: Lyublinskaya OG; Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr. 4, St. Petersburg 94064, Russia. Electronic address: o.lyublinskaya@mail.ru., Zenin VV; Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr. 4, St. Petersburg 94064, Russia. Electronic address: vvzenin@yandex.ru., Shatrova AN; Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr. 4, St. Petersburg 94064, Russia. Electronic address: shatrova@mail.ru., Aksenov ND; Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr. 4, St. Petersburg 94064, Russia. Electronic address: aksenovn@gmail.com., Zemelko VI; Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr. 4, St. Petersburg 94064, Russia. Electronic address: vzemelko@mail.ru., Domnina AP; Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr. 4, St. Petersburg 94064, Russia. Electronic address: aldomnina@mail.ru., Litanyuk AP; Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr. 4, St. Petersburg 94064, Russia. Electronic address: anyasun@mail.ru., Burova EB; Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr. 4, St. Petersburg 94064, Russia. Electronic address: lenbur87@mail.ru., Gubarev SS; Department of Medical Physics and Bioengineering, Institute of Physics, Nanotechnology and Telecommunications, St. Petersburg State Polytechnical University, Polytechnicheskaya st. 29, St. Petersburg 195251, Russia. Electronic address: svyatoslav_gubarev@mail.ru., Negulyaev YA; Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr. 4, St. Petersburg 94064, Russia; Department of Medical Physics and Bioengineering, Institute of Physics, Nanotechnology and Telecommunications, St. Petersburg State Polytechnical University, Polytechnicheskaya st. 29, St. Petersburg 195251, Russia. Electronic address: yurineg@mail.cytspb.rssi.ru., Nikolsky NN; Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr. 4, St. Petersburg 94064, Russia; Department of Medical Physics and Bioengineering, Institute of Physics, Nanotechnology and Telecommunications, St. Petersburg State Polytechnical University, Polytechnicheskaya st. 29, St. Petersburg 195251, Russia. Electronic address: nikolsky@mail.cytspb.rssi.ru.
Jazyk: angličtina
Zdroj: Free radical biology & medicine [Free Radic Biol Med] 2014 Oct; Vol. 75, pp. 60-8. Date of Electronic Publication: 2014 Jul 15.
DOI: 10.1016/j.freeradbiomed.2014.07.008
Abstrakt: Hydroethidine (HE) is a blue fluorescent dye that is intracellularly converted into red-emitting products on two-electron oxidation. One of these products, namely 2-hydroxyethidium, is formed as the result of HE superoxide anion-specific oxidation, and so HE is widely used for the detection of superoxide in cells and tissues. In our experiments we exploited three cell lines of different origin: K562 (human leukemia cells), A431 (human epidermoid carcinoma cells), and SCE2304 (human mesenchymal stem cells derived from endometrium). Using fluorescent microscopy and flow cytometry analysis, we showed that HE intracellular oxidation products accumulate mostly in the cell mitochondria. This accumulation provokes gradual depolarization of mitochondrial membrane, affects oxygen consumption rate in HE-treated cells, and causes cellular apoptosis in the case of high HE concentrations and/or long cell incubations with HE, as well as a high rate of HE oxidation in cells exposed to some stimuli.
(Copyright © 2014 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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