Genetic evaluation of mesenchymal stem cells by G-banded karyotyping in a Cell Technology Center.

Autor: Borgonovo T; Pontifícia Universidade Católica do Paraná (PUC-PR), Curitiba, PR, Brazil. Electronic address: tamaraborgonovo@yahoo.com.br., Vaz IM; Pontifícia Universidade Católica do Paraná (PUC-PR), Curitiba, PR, Brazil., Senegaglia AC; Pontifícia Universidade Católica do Paraná (PUC-PR), Curitiba, PR, Brazil., Rebelatto CL; Pontifícia Universidade Católica do Paraná (PUC-PR), Curitiba, PR, Brazil., Brofman PR; Pontifícia Universidade Católica do Paraná (PUC-PR), Curitiba, PR, Brazil.
Jazyk: angličtina
Zdroj: Revista brasileira de hematologia e hemoterapia [Rev Bras Hematol Hemoter] 2014 May-Jun; Vol. 36 (3), pp. 202-7. Date of Electronic Publication: 2014 Apr 03.
DOI: 10.1016/j.bjhh.2014.03.006
Abstrakt: Objective: To present the initial results of first three years of implementation of a genetic evaluation test for bone marrow-derived mesenchymal stem cells in a Cell Technology Center.
Methods: A retrospective study was carried out of 21 candidates for cell therapy. After the isolation of bone marrow mononuclear cells by density gradient, mesenchymal stem cells were cultivated and expanded at least until the second passage. Cytogenetic analyses were performed before and after cell expansion (62 samples) using G-banded karyotyping.
Results: All the samples analyzed, before and after cell expansion, had normal karyotypes, showing no clonal chromosomal changes. Signs of chromosomal instability were observed in 11 out of 21 patients (52%). From a total of 910 analyzed metaphases, five chromatid gaps, six chromatid breaks and 14 tetraploid cells were detected giving as total of 25 metaphases with chromosome damage (2.75%).
Conclusion: The absence of clonal chromosomal aberrations in our results for G-banded karyotyping shows the maintenance of chromosomal stability of bone marrow-derived mesenchymal stem cells until the second passage; however, signs of chromosomal instability such as chromatid gaps, chromosome breaks and tetraploidy indicate that the long-term cultivation of these cells can provide an intermediate step for tumorigenesis.
(Copyright © 2014 Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular. Published by Elsevier Editora Ltda. All rights reserved.)
Databáze: MEDLINE
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