| Autor: |
Vuorinen PI; Department of Clinical Microbiology, Tampere, University Central Hospital, Finland., Soppi ET, Laine ST, Mörsky PJ |
| Jazyk: |
angličtina |
| Zdroj: |
American journal of clinical pathology [Am J Clin Pathol] 1989 Jul; Vol. 92 (1), pp. 93-6. |
| DOI: |
10.1093/ajcp/92.1.93 |
| Abstrakt: |
The Behring Nephelometer is a new automatic analyzer designed for the measurement of specific proteins. The authors evaluated the nephelometer in the determination of M proteins as compared with immunodiffusion and cellulose acetate electrophoresis. In addition, the effects of sample dilution and antigen excess on the nephelometric measurement of M proteins were studied in 24 patient samples containing a single monoclonal IgG, IgA, or IgM M component. Immunodiffusion cannot be recommended for the determination of M proteins. Nephelometry and protein electrophoresis gave similar results with few exceptions. Sera with immunoglobulin monomer-oligomer pairs involve difficulties in serum electrophoresis. In certain cases, special care must be taken to adjust the nephelometric measurement of the M protein to the most accurate part of the calibration curve. All in all, the new Behring Nephelometer proved precise and reliable in M protein determination without risk of antigen excess. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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