Influence of Populus genotype on gene expression by the wood decay fungus Phanerochaete chrysosporium.

Autor:	Gaskell J; USDA Forest Service, Forest Products Laboratory, Madison, Wisconsin, USA., Marty A; Department of Bacteriology, University of Wisconsin, Madison, Madison, Wisconsin, USA., Mozuch M; USDA Forest Service, Forest Products Laboratory, Madison, Wisconsin, USA., Kersten PJ; USDA Forest Service, Forest Products Laboratory, Madison, Wisconsin, USA., Splinter BonDurant S; Genetics and Biotechnology Center, University of Wisconsin, Madison, Madison, Wisconsin, USA., Sabat G; Genetics and Biotechnology Center, University of Wisconsin, Madison, Madison, Wisconsin, USA., Azarpira A; Department of Biochemistry, and Department of Energy Great Lakes Bioenergy Research Center, University of Wisconsin, Madison, Madison, Wisconsin, USA., Ralph J; Department of Biochemistry, and Department of Energy Great Lakes Bioenergy Research Center, University of Wisconsin, Madison, Madison, Wisconsin, USA., Skyba O; Department of Wood Science, University of British Columbia, Vancouver, British Columbia, Canada., Mansfield SD; Department of Wood Science, University of British Columbia, Vancouver, British Columbia, Canada., Blanchette RA; Department of Plant Pathology, University of Minnesota, Saint Paul, Minnesota, USA., Cullen D; USDA Forest Service, Forest Products Laboratory, Madison, Wisconsin, USA dcullen@wisc.edu.
Jazyk:	angličtina
Zdroj:	Applied and environmental microbiology [Appl Environ Microbiol] 2014 Sep; Vol. 80 (18), pp. 5828-35. Date of Electronic Publication: 2014 Jul 11.
DOI:	10.1128/AEM.01604-14
Abstrakt:	We examined gene expression patterns in the lignin-degrading fungus Phanerochaete chrysosporium when it colonizes hybrid poplar (Populus alba × tremula) and syringyl (S)-rich transgenic derivatives. A combination of microarrays and liquid chromatography-tandem mass spectrometry (LC-MS/MS) allowed detection of a total of 9,959 transcripts and 793 proteins. Comparisons of P. chrysosporium transcript abundance in medium containing poplar or glucose as a sole carbon source showed 113 regulated genes, 11 of which were significantly higher (>2-fold, P < 0.05) in transgenic line 64 relative to the parental line. Possibly related to the very large amounts of syringyl (S) units in this transgenic tree (94 mol% S), several oxidoreductases were among the upregulated genes. Peptides corresponding to a total of 18 oxidoreductases were identified in medium consisting of biomass from line 64 or 82 (85 mol% S) but not in the parental clone (65 mol% S). These results demonstrate that P. chrysosporium gene expression patterns are substantially influenced by lignin composition. (Copyright © 2014, American Society for Microbiology. All Rights Reserved.)
Databáze:	MEDLINE
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