Parainfluenza virus type 1 induces epithelial IL-8 production via p38-MAPK signalling.

Autor: Galván Morales MÁ; Department of Virology and Mycology Research, National Institute of Respiratory Diseases 'Ismael Cosio Villegas', Calzada de Tlalpan 4502, Colonia Sección XVI, Delegación Tlalpan, 14080 México, DF, Mexico., Cabello Gutiérrez C; Department of Virology and Mycology Research, National Institute of Respiratory Diseases 'Ismael Cosio Villegas', Calzada de Tlalpan 4502, Colonia Sección XVI, Delegación Tlalpan, 14080 México, DF, Mexico., Mejía Nepomuceno F; Department of Virology and Mycology Research, National Institute of Respiratory Diseases 'Ismael Cosio Villegas', Calzada de Tlalpan 4502, Colonia Sección XVI, Delegación Tlalpan, 14080 México, DF, Mexico., Valle Peralta L; Department of Virology and Mycology Research, National Institute of Respiratory Diseases 'Ismael Cosio Villegas', Calzada de Tlalpan 4502, Colonia Sección XVI, Delegación Tlalpan, 14080 México, DF, Mexico., Valencia Maqueda E; Department of Virology and Mycology Research, National Institute of Respiratory Diseases 'Ismael Cosio Villegas', Calzada de Tlalpan 4502, Colonia Sección XVI, Delegación Tlalpan, 14080 México, DF, Mexico., Manjarrez Zavala ME; Department of Virology and Mycology Research, National Institute of Respiratory Diseases 'Ismael Cosio Villegas', Calzada de Tlalpan 4502, Colonia Sección XVI, Delegación Tlalpan, 14080 México, DF, Mexico.
Jazyk: angličtina
Zdroj: Journal of immunology research [J Immunol Res] 2014; Vol. 2014, pp. 515984. Date of Electronic Publication: 2014 Jun 11.
DOI: 10.1155/2014/515984
Abstrakt: Human parainfluenza virus type 1 (HPIV-1) is the most common cause of croup in infants. The aim of this study was to describe molecular mechanisms associated with IL-8 production during HPIV-1 infection and the role of viral replication in MAPK synthesis and activation. An in vitro model of HPIV-1 infection in the HEp-2 and A549 cell lines was used; a kinetic-based ELISA for IL-8 detection was also used, phosphorylation of the mitogen-activated protein kinases (MAPKs) was identified by Western blot analysis, and specific inhibitors for each kinase were used to identify which MAPK was involved. Inactivated viruses were used to assess whether viral replication is required for IL-8 production. Results revealed a gradual increase in IL-8 production at different selected times, when phosphorylation of MAPK was detected. The secretion of IL-8 in the two cell lines infected with the HPIV-1 is related to the phosphorylation of the MAPK as well as viral replication. Inhibition of p38 suppressed the secretion of IL-8 in the HEp-2 cells. No kinase activation was observed when viruses were inactivated.
Databáze: MEDLINE
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