Endothelial Nitric Oxide Synthase Gene T-786C Polymorphism in Renal Transplant Recipients.

Autor:	Azarpira N; Shiraz Transplant Research Center, University of Medical Sciences, Shiraz, Iran., Geramizadeh B; Shiraz Transplant Research Center, University of Medical Sciences, Shiraz, Iran., Nikeghbalian S; Shiraz Transplant Research Center, University of Medical Sciences, Shiraz, Iran ; Organ Transplant Center, Shiraz , University of Medical Sciences, Shiraz, Iran., Bahador A; Shiraz Transplant Research Center, University of Medical Sciences, Shiraz, Iran., Yaghobi R; Shiraz Transplant Research Center, University of Medical Sciences, Shiraz, Iran., Karimi H; Shiraz Transplant Research Center, University of Medical Sciences, Shiraz, Iran., Ayatolahi M; Shiraz Transplant Research Center, University of Medical Sciences, Shiraz, Iran., Aghdai MH; Shiraz Transplant Research Center, University of Medical Sciences, Shiraz, Iran., Salahi H; Organ Transplant Center, Shiraz , University of Medical Sciences, Shiraz, Iran., Malek-Hosseini SA; Shiraz Transplant Research Center, University of Medical Sciences, Shiraz, Iran ; Organ Transplant Center, Shiraz , University of Medical Sciences, Shiraz, Iran., Roozbeh J; Organ Transplant Center, Shiraz , University of Medical Sciences, Shiraz, Iran., Sagheb M; Organ Transplant Center, Shiraz , University of Medical Sciences, Shiraz, Iran., Raisjalali GH; Organ Transplant Center, Shiraz , University of Medical Sciences, Shiraz, Iran., Behzadi A; Organ Transplant Center, Shiraz , University of Medical Sciences, Shiraz, Iran.
Jazyk:	angličtina
Zdroj:	International journal of organ transplantation medicine [Int J Organ Transplant Med] 2011; Vol. 2 (2), pp. 87-92.
Abstrakt:	Background: Nitric oxide (NO) is a major mediator in vascular biology, regulating regional blood flow. NO and the enzymes required for its production contribute to ischemia-reperfusion injury. The T-786C functional polymorphism in the promoter region substantially reduces promoter activity of the endothelial nitric oxide synthase (eNOS) gene and compromises endothelial NO synthesis. Objective: To examine the association between T-786C (rs 2070744) single nucleotide polymorphism (SNP) in eNOS gene and the development of acute rejection in renal transplant patients. Methods: 60 renal transplant recipients (30 with episodes of acute rejection (ARs) and 30 without rejection (non-ARs)), between June 2008 and March 2010, were included in this study. The polymorphism was determined by PCR-restriction fragment-length polymorphism analysis. Results: The distribution of the genotypes were TT/TC/CC 60%, 33.4%, 6.6%, and 43%, 46.7%, 13.3% in ARs and non-ARs, respectively (p=0.28). The frequency of T-allele was 76.7% and 66.3%; and for C-allele was 66.6% and 33.3% in ARs and non-ARs, respectively (p=0.09). There were no significant associations between these polymorphisms and acute and chronic kidney allograft rejection. Conclusion: We could not detect any significant association between polymorphism in T-786C of eNOS gene and the development of acute rejection.
Databáze:	MEDLINE
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