[Evaluation of the VITEK 2 system (AST-YSO1 cards) for antifungal susceptibility testing against different Candida species].

Autor:	Ochiuzzi ME; Sección Microbiología Laboratorio Central, Hospital General de Agudos Dr. C.G. Durand, Ciudad Autónoma de Buenos Aires, Argentina. Electronic address: omarieu@hotmail.com., Arechavala A; Red de Micología CABA, Argentina., Guelfand L; Red de Micología CABA, Argentina., Maldonado I; Red de Micología CABA, Argentina., Soloaga R; Hospital Naval Pedro Mallo, Ciudad Autónoma de Buenos Aires, Argentina.
Jazyk:	Spanish; Castilian
Zdroj:	Revista Argentina de microbiologia [Rev Argent Microbiol] 2014 Apr-Jun; Vol. 46 (2), pp. 111-8.
DOI:	10.1016/S0325-7541(14)70058-6
Abstrakt:	The aim of this investigation was to evaluate the results of antifungal susceptibility for various Candida species using the Vitek 2 semi-automated system (AST-YSO1 cards, bioMérieux), and to compare them with those obtained by the CLSI (Clinical and Laboratory Standards Institute) broth microdilution reference method (Document M27-A3,2008). The essential agreement (EA) was > 90%, except for Candida glabrata against voriconazole (VCZ); and for Candida krusei against fluconazole (FCZ). The overall categorical agreement (CA) was > 90% when FCZ was evaluated and 89.5% at 24h and 80.7% at 48 h for VCZ. The average time for obtaining results was 15.5h. Minor errors were 7.8% at 24h and 6.1% at 48 h for FCZ, and 10.5% at 24h and 19.3% at 48 h for VCZ. There was only one very major error for FCZ against Candida parapsilosis and no major errors were observed. For amphotericin B, only three isolates showed MICs ≥ 2 μg/ml. The Vitek 2 system detected the MIC value for various Candida species and showed excellent agreement with the reference method proposed by the CLSI. (Copyright © 2014 Asociación Colombiana de Psiquiatría. Publicado por Elsevier España. All rights reserved.)
Databáze:	MEDLINE
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