| Autor: |
Serebrijski IG; Institute of Genetics and Selection of Industrial Microorganisms, Moscow, U.S.S.R., Kazakova SM, Tsygankov YD |
| Jazyk: |
angličtina |
| Zdroj: |
FEMS microbiology letters [FEMS Microbiol Lett] 1989 May; Vol. 50 (1-2), pp. 203-6. |
| DOI: |
10.1016/0378-1097(89)90486-2 |
| Abstrakt: |
The transposon-loaded plasmid pAS8-121, incapable of autonomous replication in Gram-negative bacteria of non-enteric group, was transferred to Methylobacillus flagellatum KT wild type strain MFK1. The transconjugants arose at a frequency of 10(-7) per donor cell. The majority of the transconjugants tested exhibited the transfer of all selected chromosomal markers at rather high (10(-4)-10(-6) per donor cell) but similar frequencies. Only one of the obtained donors, designated MFK 64, was capable of mobilizing M. flagellatum KT chromosome in a polarized manner. The integrated nature of the plasmid in this and other MFK1 (pAS8-121) derivatives was supported by the results of DNA-DNA hybridization. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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