Nanometer scale titanium surface texturing are detected by signaling pathways involving transient FAK and Src activations.

Autor: Zambuzzi WF; Departmento de Química e Bioquímica, Instituto de Biociências, Universidade Estadual Paulista - UNESP, Botucatu, São Paulo, Brazil., Bonfante EA; Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, São Paulo, Brazil., Jimbo R; Department of Prosthodontics, Faculty of Odontology, Malmö University, Malmö, Sweden., Hayashi M; Department of Prosthodontics, Faculty of Odontology, Malmö University, Malmö, Sweden., Andersson M; Department of Chemical and Biological Engineering, Applied Surface Chemistry, Chalmers University of Technology, Gothenburg, Sweden., Alves G; Department of Cell and Molecular Biology, Institute of Biology, Universidade Federal Fluminense, Niteroi, Brazil., Takamori ER; Excellion Biomedical Services, Petrópolis, Rio de Janeiro, Brazil., Beltrão PJ; National Institute of Metrology, Quality and Technology - INMETRO, Xerém, Rio de Janeiro, Brazil., Coelho PG; Department of Biomaterials and Biomimetics/Director for Research Department of Periodontology and Implant Dentistry, New York University College of Dentistry, New York, New York, United States of America., Granjeiro JM; National Institute of Metrology, Quality and Technology - INMETRO, Xerém, Rio de Janeiro, Brazil.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2014 Jul 07; Vol. 9 (7), pp. e95662. Date of Electronic Publication: 2014 Jul 07 (Print Publication: 2014).
DOI: 10.1371/journal.pone.0095662
Abstrakt: Background: It is known that physico/chemical alterations on biomaterial surfaces have the capability to modulate cellular behavior, affecting early tissue repair. Such surface modifications are aimed to improve early healing response and, clinically, offer the possibility to shorten the time from implant placement to functional loading. Since FAK and Src are intracellular proteins able to predict the quality of osteoblast adhesion, this study evaluated the osteoblast behavior in response to nanometer scale titanium surface texturing by monitoring FAK and Src phosphorylations.
Methodology: Four engineered titanium surfaces were used for the study: machined (M), dual acid-etched (DAA), resorbable media microblasted and acid-etched (MBAA), and acid-etch microblasted (AAMB). Surfaces were characterized by scanning electron microscopy, interferometry, atomic force microscopy, x-ray photoelectron spectroscopy and energy dispersive X-ray spectroscopy. Thereafter, those 4 samples were used to evaluate their cytotoxicity and interference on FAK and Src phosphorylations. Both Src and FAK were investigated by using specific antibody against specific phosphorylation sites.
Principal Findings: The results showed that both FAK and Src activations were differently modulated as a function of titanium surfaces physico/chemical configuration and protein adsorption.
Conclusions: It can be suggested that signaling pathways involving both FAK and Src could provide biomarkers to predict osteoblast adhesion onto different surfaces.
Databáze: MEDLINE
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