Cationic surfactant-based colorimetric detection of Plasmodium lactate dehydrogenase, a biomarker for malaria, using the specific DNA aptamer.
| Autor: | Lee S; Department of Chemistry, Pohang University of Science and Technology, Pohang, Gyeongbuk, Republic of Korea., Manjunatha DH; Department of Chemistry, Pohang University of Science and Technology, Pohang, Gyeongbuk, Republic of Korea., Jeon W; Department of Chemistry, Pohang University of Science and Technology, Pohang, Gyeongbuk, Republic of Korea., Ban C; Department of Chemistry, Pohang University of Science and Technology, Pohang, Gyeongbuk, Republic of Korea. |
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| Jazyk: | angličtina |
| Zdroj: | PloS one [PLoS One] 2014 Jul 03; Vol. 9 (7), pp. e100847. Date of Electronic Publication: 2014 Jul 03 (Print Publication: 2014). |
| DOI: | 10.1371/journal.pone.0100847 |
| Abstrakt: | A simple, sensitive, and selective colorimetric biosensor for the detection of the malarial biomarkers Plasmodium vivax lactate dehydrogenase (PvLDH) and Plasmodium falciparum LDH (PfLDH) was demonstrated using the pL1 aptamer as the recognition element and gold nanoparticles (AuNPs) as probes. The proposed method is based on the aggregation of AuNPs using hexadecyltrimethylammonium bromide (CTAB). The AuNPs exhibited a sensitive color change from red to blue, which could be seen directly with the naked eye and was monitored using UV-visible absorption spectroscopy and transmission electron microscopy (TEM). The reaction conditions were optimized to obtain the maximum color intensity. PvLDH and PfLDH were discernible with a detection limit of 1.25 pM and 2.94 pM, respectively. The applicability of the proposed biosensor was also examined in commercially available human serum. |
| Databáze: | MEDLINE |
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