| Autor: |
Calábria LK; Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, MG, Brazil ; Federal University of Juiz de Fora, Governador Valadares, MG, Brazil., Vieira da Costa A; Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, MG, Brazil., da Silva Oliveira RJ; Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, MG, Brazil., Ramos Deconte S; Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, MG, Brazil., Nascimento R; Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, MG, Brazil., de Carvalho WJ; Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, MG, Brazil., de Oliveira VN; Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, MG, Brazil ; Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil., Arcaro Filho CA; Department of Chemistry, Physic, and Mathematic, University of Ribeirão Preto, Ribeirão Preto, SP, Brazil., Rezende Alves de Oliveira L; Department of Chemistry, Physic, and Mathematic, University of Ribeirão Preto, Ribeirão Preto, SP, Brazil., Goulart LR; Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, MG, Brazil., Espindola FS; Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, MG, Brazil. |
| Abstrakt: |
Diabetes mellitus is a disease characterized by persistent hyperglycemia, which may lead to brain tissue damage due to oxidative stress and also contributes to neuronal death and changes in synaptic transmission. This study evaluated the effect of oxidative stress and the use of antioxidants supplementation on myosins expression levels in the brains of chronic diabetic rats induced by streptozotocin. Lipid peroxidation, antioxidant enzymes activities, and myosins-IIB and -Va expressions at transcriptional and translational levels were examined after 90 days induction. The chronic effect of the diabetes led to the upregulation of superoxide dismutase (SOD) and catalase (CAT) activities, and the downregulation of glutathione peroxidase (GPx), but there was no statistically significant increase in the malondialdehyde (MDA) levels. These alterations were accompanied by high myosin-IIB and low myosin-Va expressions. Although the antioxidant supplementation did not interfere on MDA levels, the oxidative stress caused by chronic hyperglycemia was reduced by increasing SOD and restoring CAT and GPx activities. Interestingly, after supplementation, diabetic rats recovered only myosin-Va protein levels, without interfering on myosins mRNA levels expressed in diabetic rat brains. Our results suggest that antioxidant supplementation reduces oxidative stress and also regulates the myosins protein expression, which should be beneficial to individuals with diabetes/chronic hyperglycemia. |
