Protein detection on biotin-derivatized polyallylamine by optical microring resonators.

Autor: Ullien D, Harmsma PJ, Abdulla SM, de Boer BM, Bosma D, Sudhölter EJ, de Smet LC, Jager WF
Jazyk: angličtina
Zdroj: Optics express [Opt Express] 2014 Jun 30; Vol. 22 (13), pp. 16585-94.
DOI: 10.1364/OE.22.016585
Abstrakt: Silicon optical microring resonators (MRRs) are sensitive devices that can be used for biosensing. We present a novel biosensing platform based on the application of polyelectrolyte (PE) layers on such MRRs. The top PE layer was covalently labeled with biotin to ensure binding sites for antibodies via a streptavidin-biotin binding scheme. Monitoring the shift in the microring resonance wavelength allows real-time, highly sensitive detection of the biomolecular interaction.
Databáze: MEDLINE