Abstrakt: |
The multiplication rates of 70 porcine Escherichia coli strains were compared in minimal medium and in medium supplemented with aspartic acid, lysine, serine and threonine, which were the amino acids taken up during multiplication of porcine E. coli in a complex medium. The effects of these amino acids singly or in combinations and the amino acids norleucine and norvaline on the growth of porcine E. coli were studied. Together, aspartic acid, threonine, serine and lysine increased the multiplication rates of 42.9% of the strains, an effect traced to aspartic acid, but they had no effect on an equal number of strains. The rest were inhibited, and this effect was traced to serine. Cysteine, threonine, leucine and phenylalanine singly inhibited some or all of the strains tested. Norleucine and to a lesser extent, norvaline greatly prolonged the lag phase of culture in minimal medium. The inhibitory effect of norleucine was reversed by only methionine, although isoleucine, leucine and valine which were more effective in norvaline inhibition, also showed limited antagonism to norleucine inhibition. |