[Giardia duodenalis genotypes found in the Instituto Colombiano de Bienestar Familiar day care centers and dogs in Ibagué, Colombia].

Autor:	Rodríguez V; Facultad de Medicina Veterinaria y Zootecnia, Universidad del Tolima, Ibagué, Colombia., Espinosa O; Laboratorio de Investigaciones en Parasitología Tropical, Facultad de Ciencias, Departamento de Biología, Universidad del Tolima, Ibagué, Colombia., Carranza JC; Laboratorio de Investigaciones en Parasitología Tropical, Facultad de Ciencias, Departamento de Biología, Universidad del Tolima, Ibagué, Colombia., Duque S; Grupo de Parasitología, Subdirección de Investigación Científica y Tecnológica, Dirección de Investigación en Salud Pública, Instituto Nacional de Salud, Bogotá, D.C, Colombia., Arévalo A; Grupo de Parasitología, Subdirección de Investigación Científica y Tecnológica, Dirección de Investigación en Salud Pública, Instituto Nacional de Salud, Bogotá, D.C, Colombia., Clavijo JA; Departamento de Matemáticas y Estadística, Facultad de Ciencias, Universidad del Tolima, Ibagué, Colombia., Urrea DA; Laboratorio de Investigaciones en Parasitología Tropical, Facultad de Ciencias, Departamento de Biología, Universidad del Tolima, Ibagué, Colombia., Vallejo GA; Laboratorio de Investigaciones en Parasitología Tropical, Facultad de Ciencias, Departamento de Biología, Universidad del Tolima, Ibagué, Colombia.
Jazyk:	Spanish; Castilian
Zdroj:	Biomedica : revista del Instituto Nacional de Salud [Biomedica] 2014 Apr-Jun; Vol. 34 (2), pp. 271-81.
DOI:	10.1590/S0120-41572014000200013
Abstrakt:	Introduction: Eight Giardia duodenalis genotypes (A-H) have been described to date. Genotypes A and B have been isolated from humans and a wide range of mammals; however, genotypes C-H have shown greater host specificity. Objective: Identifying G. duodenalis genotypes from cysts in faeces obtained from children attending the Instituto Colombiano de Bienestar Familiar (ICBF) day care centres and from dogs in Ibagué by PCR-RFLP targeting both the b -giardin and glutamate dehydrogenase genes. Materials and Methods: Cysts from G. duodenalis positive samples were concentrated, DNA was extracted and the b -giardin and glutamate dehydrogenase genes were analysed by PCR-RFLP. The MHOM/CO/04/G40 strain was used as positive control (this was obtained from the Grupo de Parasitología at the Instituto Nacional de Salud ). Results: Of the total human samples, 11/23 (48%) were genotyped as A and 12/23 (52%) as B; PCR-RFLP revealed that four canine samples were genotypes C and D, these being host-specific. Conclusions: Only genotypes associated with human infection (AII, BIII and BIV) were found in the children and host-specific genotypes were observed in canines (C and D). No interaction could be established between animal and human transmission cycles due to the small canine sample size and as the former did not come into contact with children attending ICBF day-care centres.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu