| Autor: |
dos-Santos PB; Keizo Asami Immunopathology Laboratory, Federal University of Pernambuco Recife, Pernambuco, 50670-901, Brazil., Zanetti JS; Department of Pathology, Ribeirão Preto Medical School, University of São Paulo Ribeirão Preto, São Paulo, 14049-900, Brazil., Vieira-de-Mello GS; Keizo Asami Immunopathology Laboratory, Federal University of Pernambuco Recife, Pernambuco, 50670-901, Brazil., Rêgo MB; Keizo Asami Immunopathology Laboratory, Federal University of Pernambuco Recife, Pernambuco, 50670-901, Brazil ; Department of Biochemistry, Biology Sciences Centre, Federal University of Pernambuco Recife, Pernambuco, 50670-901, Brazil., Ribeiro-Silva A A; Department of Pathology, Ribeirão Preto Medical School, University of São Paulo Ribeirão Preto, São Paulo, 14049-900, Brazil., Beltrão EI; Keizo Asami Immunopathology Laboratory, Federal University of Pernambuco Recife, Pernambuco, 50670-901, Brazil ; Department of Biochemistry, Biology Sciences Centre, Federal University of Pernambuco Recife, Pernambuco, 50670-901, Brazil. |
| Abstrakt: |
Increased sialylation and β1,6-branched oligosaccharides has been associated with a variety of structural changes in cell surface carbohydrates, most notably in tumorigenesis. Lectins are defined as proteins that preferentially recognize and bind carbohydrate complexes protruding from glycolipids and glycoproteins. This interaction with carbohydrates can be as specific as the interaction between antigen and antibody. Due to this type of interaction lectins have been used as experimental auxiliary tools in histopathological diagnosis of cancer. This study was designed to evaluate the differential expression of sialic acids and β1,6-N-acetylglucosaminyltransferase V (MGAT5) in invasive (IDC) and in situ (DCIS) ductal carcinoma of the breast and its possible application as prognostic biomarkers. A possible transition between pre-malign and malign lesions was evaluated using DCIS samples. Biopsies were analyzed regarding the expression of MUC1, p53, Ki-67, estrogen receptor, progesterone receptor, HER-2 and MGAT5. α2,6-linked sialic acids residues recognized by SNA lectin was overexpressed in 33.3% of IDC samples and it was related with Ki-67 (p=0.042), PR (p=0.029), lymphnodes status (p=0.017) and death (p=0.011). Regarding survival analysis SNA was the only lectin able to correlate with specific-disease survival and disease-free survival (p=0.024 and p=0.041, respectively), besides, it presents itself as an independent variable by Cox Regression analysis (p= 0.004). Comparing IDC and DCIS cases, only SNA showed different staining pattern (p=0.034). The presence of sialic acids on tumor cell surface can be an indicative of poor prognosis and our study provides further evidence that SNA lectin can be used as a prognostic probe in IDC and DCIS patients. |
