| Autor: |
Chung-Davidson YW; Department of Fisheries & Wildlife, Michigan State University; chungyuw@msu.edu., Davidson PJ; Department of Fisheries & Wildlife, Michigan State University., Scott AM; Department of Fisheries & Wildlife, Michigan State University., Walaszczyk EJ; Department of Fisheries & Wildlife, Michigan State University., Brant CO; Department of Fisheries & Wildlife, Michigan State University., Buchinger T; Department of Fisheries & Wildlife, Michigan State University., Johnson NS; Hammond Bay Biological Station, Great Lakes Science Center, U.S. Geological Survey., Li W; Department of Fisheries & Wildlife, Michigan State University. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of visualized experiments : JoVE [J Vis Exp] 2014 Jun 06 (88). Date of Electronic Publication: 2014 Jun 06. |
| DOI: |
10.3791/51648 |
| Abstrakt: |
Biliary atresia is a rare disease of infancy, with an estimated 1 in 15,000 frequency in the southeast United States, but more common in East Asian countries, with a reported frequency of 1 in 5,000 in Taiwan. Although much is known about the management of biliary atresia, its pathogenesis is still elusive. The sea lamprey (Petromyzon marinus) provides a unique opportunity to examine the mechanism and progression of biliary degeneration. Sea lamprey develop through three distinct life stages: larval, parasitic, and adult. During the transition from larvae to parasitic juvenile, sea lamprey undergo metamorphosis with dramatic reorganization and remodeling in external morphology and internal organs. In the liver, the entire biliary system is lost, including the gall bladder and the biliary tree. A newly-developed method called "CLARITY" was modified to clarify the entire liver and the junction with the intestine in metamorphic sea lamprey. The process of biliary degeneration was visualized and discerned during sea lamprey metamorphosis by using laser scanning confocal microscopy. This method provides a powerful tool to study biliary atresia in a unique animal model. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
