| Autor: |
Barbaro E; Department of Environmental Sciences, Informatics and Statistics, University of Venice, Ca' Foscari, Calle Larga Santa Marta 2137, 30123, Venice, Italy, barbaro@unive.it., Zangrando R, Vecchiato M, Turetta C, Barbante C, Gambaro A |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2014 Sep; Vol. 406 (22), pp. 5259-70. Date of Electronic Publication: 2014 Jun 25. |
| DOI: |
10.1007/s00216-014-7961-y |
| Abstrakt: |
Amino acids represent a fraction of organic matter in marine and freshwater ecosystems, and a source of carbon, nitrogen and energy. L-Amino acids are the most common enantiomers in nature because these chiral forms are used during the biosynthesis of proteins and peptide. To the contrary, the occurrence of D-amino acids is usually linked to the presence of bacteria. We investigated the distribution of L- and D-amino acids in the lacustrine environment of Terra Nova Bay, Antarctica, in order to define their natural composition in this area and to individuate a possible relationship with primary production. A simultaneous chromatographic separation of 40 L- and D-amino acids was performed using a chiral stationary phase based on teicoplainin aglycone (CHIROBIOTIC TAG). The chromatographic separation was coupled to two different mass spectrometers--an LTQ-Orbitrap XL (Thermo Fisher Scientific) and an API 4000 (ABSciex)--in order to investigate their quantitative performance. High-performance liquid chromatography coupled with mass spectrometry methods were evaluated through the estimation of their linear ranges, repeatability, accuracy and detection and quantification limits. The high-resolution mass spectrometer LTQ-Orbitrap XL presented detection limits between 0.4 and 7 μg L (-1), while the triple quadrupole mass spectrometer API 4000 achieved the best detection limits reported in the literature for the quantification of amino acids (between 4 and 200 ng L (-1)). The most sensitive method, HPLC-API 4000, was applied to lake water samples. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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