Sensitivity and specificity of serological tests, histopathology and immunohistochemistry for detection of Toxoplasma gondii infection in domestic chickens.

Autor: Casartelli-Alves L; Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Av. Brasil, 4365, Rio de Janeiro, RJ CEP: 21040-360, Brazil., Boechat VC; Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Av. Brasil, 4365, Rio de Janeiro, RJ CEP: 21040-360, Brazil., Macedo-Couto R; Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Av. Brasil, 4365, Rio de Janeiro, RJ CEP: 21040-360, Brazil., Ferreira LC; Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Av. Brasil, 4365, Rio de Janeiro, RJ CEP: 21040-360, Brazil., Nicolau JL; Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil, 4365, Rio de Janeiro, RJ CEP: 21040-360, Brazil., Neves LB; Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil, 4365, Rio de Janeiro, RJ CEP: 21040-360, Brazil., Millar PR; Instituto Biomédico, Universidade Federal Fluminense, Rua Professor Ernani Melo, 101, Niterói, RJ CEP: 24210-130, Brazil., Vicente RT; Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil, 4365, Rio de Janeiro, RJ CEP: 21040-360, Brazil., Oliveira RV; Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Av. Brasil, 4365, Rio de Janeiro, RJ CEP: 21040-360, Brazil., Muniz AG; Instituto Nacional do Câncer, Praça da Cruz Vermelha, 23, Rio de Janeiro, RJ CEP: 20230-130, Brazil., Bonna IC; Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Av. Brasil, 4365, Rio de Janeiro, RJ CEP: 21040-360, Brazil., Amendoeira MR; Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil, 4365, Rio de Janeiro, RJ CEP: 21040-360, Brazil., Silva RC; College of Veterinary Medicine, Mississippi State University, 240 Wise Center Drive, 39762 Starkville, USA., Langoni H; Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista Júlio de Mesquita Filho, Botucatu, SP CEP: 18618-970, Brazil., Schubach TM; Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Av. Brasil, 4365, Rio de Janeiro, RJ CEP: 21040-360, Brazil., Menezes RC; Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Av. Brasil, 4365, Rio de Janeiro, RJ CEP: 21040-360, Brazil. Electronic address: rodrigo.menezes@ipec.fiocruz.br.
Jazyk: angličtina
Zdroj: Veterinary parasitology [Vet Parasitol] 2014 Aug 29; Vol. 204 (3-4), pp. 346-51. Date of Electronic Publication: 2014 Jun 04.
DOI: 10.1016/j.vetpar.2014.05.039
Abstrakt: Since free-range chickens are important for the epidemiology of toxoplasmosis, this study evaluated the sensitivity and specificity of different laboratory techniques for the diagnosis of Toxoplasma gondii in these animals. Serum samples from 135 adult domestic chickens were tested for anti-T. gondii antibodies by the indirect fluorescent antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA), modified agglutination test (MAT), and indirect hemagglutination test (IHAT). Tissue samples from all animals were analyzed by histopathology, immunohistochemistry and mouse bioassay (gold standard). Fifty-four chickens were positive for T. gondii in the bioassay. The sensitivity and specificity of the different tests were, respectively, 85% and 56% for ELISA; 80% and 52% for IFAT; 76% and 68% for MAT; 61% and 80% for IHAT; 7% and 98% for immunohistochemistry, and 6% and 98% for histopathology. The MAT was the most effective method for the diagnosis of T. gondii infection in chickens, followed by ELISA. Histopathology and immunohistochemistry are useful tools for the diagnosis of T. gondii infection in chickens due to their specificity.
(Copyright © 2014 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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